Dataset Information

Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach

ABSTRACT: Clostridium cellulovorans is among the most promising candidates for consolidated bioprocessing (CBP) of cellulosic biomass to liquid biofuels (e.g. ethanol, butanol). C. cellulovoranscan metabolize all the main plant polysaccharides (i.e. cellulose, hemicellusoses and pectins). Unlike other well established cellulolytic microorganisms, C. cellulovorans most abundant catabolite is butyrate. This attracted attention on this strain as potential butanol producer since most reactions involved in butyrate and butanol synthetic pathway from acetyl-coA are common. Recent studies demonstrated that the introduction of a single heterologous alcohol/aldehyde dehydrogenase can significantly divert the branching-point intermediate, i.e. butyryl-CoA, towards butanol production in this strain. In spite of the potential of C. cellulovorans for application in CBP of plant biomass, engineering its metabolic pathways towards industrial utilization still requires enhanced understanding of its metabolism. Few recent studies aimed at understanding the regulation of C. cellulovorans central carbon metabolism in response to different substrate availability, which seem insufficient for the aforementioned purposes. The present study aimed at improving comprehension of cellulose metabolism in C. cellulovorans by comparing growth kinetics, substrate consumption/product accumulation and whole-cell soluble proteome with those of C. cellulovorans grown on a soluble carbohydrate, i.e. glucose, as the main carbon source. Modulations of the central carbon metabolism in response to changes in the growth substrate were detected, including the regulation of glycolytic enzymes, fermentation pathways and nitrogen assimilation. Our data suggest that a higher energy expenditure occurs in cellulose-grown C. cellulovorans which induces up-regulation of ATP synthetic pathways, e.g. acetate production and ATP synthase.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Clostridium Cellulovorans 743b

SUBMITTER: Marcello Manfredi

LAB HEAD: Marcello Manfredi

PROVIDER: PXD015487 | Pride | 2020-01-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Mazzoli_repl1_AVICEL_2ug_LIB1.dat	Other
	Mazzoli_repl1_AVICEL_2ug_LIB2.dat	Other
	Mazzoli_repl1_GLUCOSIO_2ug_LIB1.dat	Other
	Mazzoli_repl1_GLUCOSIO_2ug_LIB2.dat	Other
	Mazzoli_repl2_AVICEL_2ug_LIB1.dat	Other

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Publications

Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach.

Usai Giulia G Cirrincione Simona S Re Angela A Manfredi Marcello M Pagnani Andrea A Pessione Enrica E Mazzoli Roberto R

Journal of proteomics 20200123

Clostridium cellulovorans is among the most promising candidates for consolidated bioprocessing (CBP) of cellulosic biomass to liquid biofuels (ethanol, butanol). C. cellulovorans metabolizes all the main plant polysaccharides and mainly produces butyrate. Since most butyrate and butanol biosynthetic reactions from acetyl-CoA are common, introduction of single heterologous alcohol/aldehyde dehydrogenase can divert the branching-point intermediate (butyryl-CoA) towards butanol production in this ...[more]

PMID: 31982546

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