Proteomics

Dataset Information

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Methyltransferase METTL5 interactome

ABSTRACT: Cellular RNAs are covalently modified and these modifications can impact on all biological processes and hence are implicated in different types of diseases. Amongst RNA modifications, N6-methyladenosine (m6A) is one of the most widespread and has been found on messenger (mRNA), ribosomal (rRNA), non-coding and spliceosomal RNAs. We undertook a systematic screen to uncover new RNA-methyltransferases. We demonstrate that the methyltransferase-like 5 protein (METTL5) is an 18S rRNA specific methyltransferase and interacts specifically with Trmt122.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Epithelial Cell Of Cervix

DISEASE(S): Cervix Carcinoma

SUBMITTER: Pascal Jansen  

LAB HEAD: Robert Schneider

PROVIDER: PXD015582 | Pride | 2020-09-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20160902_Fusion1_SA_PJ_MB_RSext_HelaFRT_methylases_wce_M5_GFP1.raw Raw
20160902_Fusion1_SA_PJ_MB_RSext_HelaFRT_methylases_wce_M5_GFP2.raw Raw
20160902_Fusion1_SA_PJ_MB_RSext_HelaFRT_methylases_wce_M5_GFP3.raw Raw
20160902_Fusion1_SA_PJ_MB_RSext_HelaFRT_wt_wce_GFP1.raw Raw
20160902_Fusion1_SA_PJ_MB_RSext_HelaFRT_wt_wce_GFP2.raw Raw
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Publications

The rRNA m<sup>6</sup>A methyltransferase METTL5 is involved in pluripotency and developmental programs.

Ignatova Valentina V VV   Stolz Paul P   Kaiser Steffen S   Gustafsson Tobias H TH   Lastres Palma Rico PR   Sanz-Moreno Adrián A   Cho Yi-Li YL   Amarie Oana V OV   Aguilar-Pimentel Antonio A   Klein-Rodewald Tanja T   Calzada-Wack Julia J   Becker Lore L   Marschall Susan S   Kraiger Markus M   Garrett Lillian L   Seisenberger Claudia C   Hölter Sabine M SM   Borland Kayla K   Van De Logt Erik E   Jansen Pascal W T C PWTC   Baltissen Marijke P MP   Valenta Magdalena M   Vermeulen Michiel M   Wurst Wolfgang W   Gailus-Durner Valerie V   Fuchs Helmut H   Hrabe de Angelis Martin M   Rando Oliver J OJ   Kellner Stefanie M SM   Bultmann Sebastian S   Schneider Robert R  

Genes & development 20200326 9-10

Covalent chemical modifications of cellular RNAs directly impact all biological processes. However, our mechanistic understanding of the enzymes catalyzing these modifications, their substrates and biological functions, remains vague. Amongst RNA modifications N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is widespread and found in messenger (mRNA), ribosomal (rRNA), and noncoding RNAs. Here, we undertook a systematic screen to uncover new RNA methyltransferases. We demonstrate that the methylt  ...[more]

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