Dataset Information

The phosphoproteomic landscape of two FLT3+ Acute Myeloid Leukemia (AML) patient samples-LYSATES.

ABSTRACT: Acute myeloid leukemia (AML) is a clonal hematopoietic malignancy, characterized by expansion of immature leukemic blasts in the bone marrow. In AML, specific tyrosine kinases have been implicated in leukemogenesis, and are associated with poor treatment outcome. However, targeted therapy using kinase inhibitors (KIs) has had limited success, and may be improved by proper patient selection. We performed phosphotyrosine (pY) based, label-free phosphoproteomics to identify hyperphosphorylated, active kinases in two FLT3+ AML Pt samples and this data is deposited in PXD015639 . Here are the corresponding lysate samples

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: Sander Piersma

LAB HEAD: Connie Ramona Jimenez

PROVIDER: PXD015662 | Pride | 2020-03-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_txt.zip	Other
	Ptlysatesamplelabeling.xlsx	Xlsx
	QE1_160411_OPL1013_CvA_LYSATE_blood_patient_bench_M.raw	Raw
	QE1_160411_OPL1013_CvA_LYSATE_blood_patient_bench_N.raw	Raw
	QE1_160411_OPL1013_CvA_LYSATE_blood_patient_bench_O.raw	Raw

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Publications

Phosphotyrosine-based Phosphoproteomics for Target Identification and Drug Response Prediction in AML Cell Lines.

van Alphen Carolien C Cloos Jacqueline J Beekhof Robin R Cucchi David G J DGJ Piersma Sander R SR Knol Jaco C JC Henneman Alex A AA Pham Thang V TV van Meerloo Johan J Ossenkoppele Gert J GJ Verheul Henk M W HMW Janssen Jeroen J W M JJWM Jimenez Connie R CR

Molecular & cellular proteomics : MCP 20200226 5

Acute myeloid leukemia (AML) is a clonal disorder arising from hematopoietic myeloid progenitors. Aberrantly activated tyrosine kinases (TK) are involved in leukemogenesis and are associated with poor treatment outcome. Kinase inhibitor (KI) treatment has shown promise in improving patient outcome in AML. However, inhibitor selection for patients is suboptimal.In a preclinical effort to address KI selection, we analyzed a panel of 16 AML cell lines using phosphotyrosine (pY) enrichment-based, la ...[more]

PMID: 32102969

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