Proteomics

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Engineered fibre enables targeted activation of butyrate-producing microbiota in the distal gut


ABSTRACT: Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have engineered an acetylated galactoglucomannan (AcGGM) fibre from spruce trees to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. The accuracy of AcGGM was tested in an applied pig feeding trial, which resolved 355 metagenome-assembled genomes together with quantitative metaproteomes. In AcGGM-fed pigs, both target populations differentially expressed AcGGM-specific polysaccharide utilization loci, including novel, mannan-specific esterases that are critical to its deconstruction. We additionally observed a “butterfly effect”, whereby numerous metabolic changes and interdependent cross-feeding pathways were detected in neighboring non-mannolytic populations that produce short-chain fatty acids. Our findings show that intricate structural features and acetylation patterns of dietary fibre can be customized to specific bacterial populations, with the possibility to create greater modulatory effects at large.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Faecalibacterium Prausnitzii Roseburia Dialister Catenibacterium Coprococcus Eutactus Butyrivibrio Fibrisolvens Ruminococcus Gnavus

SUBMITTER: Magnus Arntzen  

LAB HEAD: Bjørge Westereng

PROVIDER: PXD015757 | Pride | 2020-09-28

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
180622_Sabina_1_1.raw Raw
180622_Sabina_1_2.raw Raw
180622_Sabina_1_3.raw Raw
180622_Sabina_1_4.raw Raw
180622_Sabina_1_5.raw Raw
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Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have tailored an acetylated galactoglucomannan (AcGGM) fibre to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. Here, we test the accuracy of AcGGM within the complex endogenous gut microbiome of pigs, wherein we resolve 355 metagenome-assemble  ...[more]

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