Dataset Information

Lysiphlebus fabarum (Hymenoptera: Braconidae: Aphidiinae) venom proteomic analysis

ABSTRACT: Venom injected at oviposition is crucial for successful reproduction in most parasitoid wasp species (Moreau & Asgari 2015; Poirié et al. 2014). The venom of the pea aphid parasitoid Aphidius ervi was analyzed previously using a combined transcriptomic and proteomic approach (Colinet et al. 2014) and we applied similar methods here to compare the venom composition in Lysiphlebus fabarum, that also used the pea aphid as host. Venom was extracted from the L. fabarum venom gland and analyzed using 1D gel electrophoresis and mass spectrometry. A total of 35 L. fabarum proteins were identified as putative venom proteins using these results combined with available transcriptomic data (Dennis et al. 2017) and the genomic data.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Lysiphlebus Fabarum

TISSUE(S): Venom

SUBMITTER: Maya BELGHAZI

LAB HEAD: Jean-Luc GATTI

PROVIDER: PXD015758 | Pride | 2020-04-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20152003_JLG_1DAg_Lysiphlebus_bande01.mgf	Mgf
	20152003_JLG_1DAg_Lysiphlebus_bande01.pride.mgf.gz	Mgf
	20152003_JLG_1DAg_Lysiphlebus_bande02.mgf	Mgf
	20152003_JLG_1DAg_Lysiphlebus_bande02.pride.mgf.gz	Mgf
	20152003_JLG_1DAg_Lysiphlebus_bande03.mgf	Mgf

Items per page:

1 - 5 of 80

Publications

Functional insights from the GC-poor genomes of two aphid parasitoids, Aphidius ervi and Lysiphlebus fabarum.

Dennis Alice B AB Ballesteros Gabriel I GI Robin Stéphanie S Schrader Lukas L Bast Jens J Berghöfer Jan J Beukeboom Leo W LW Belghazi Maya M Bretaudeau Anthony A Buellesbach Jan J Cash Elizabeth E Colinet Dominique D Dumas Zoé Z Errbii Mohammed M Falabella Patrizia P Gatti Jean-Luc JL Geuverink Elzemiek E Gibson Joshua D JD Hertaeg Corinne C Hartmann Stefanie S Jacquin-Joly Emmanuelle E Lammers Mark M Lavandero Blas I BI Lindenbaum Ina I Massardier-Galata Lauriane L Meslin Camille C Montagné Nicolas N Pak Nina N Poirié Marylène M Salvia Rosanna R Smith Chris R CR Tagu Denis D Tares Sophie S Vogel Heiko H Schwander Tanja T Simon Jean-Christophe JC Figueroa Christian C CC Vorburger Christoph C Legeai Fabrice F Gadau Jürgen J

BMC genomics 20200529 1

<h4>Background</h4>Parasitoid wasps have fascinating life cycles and play an important role in trophic networks, yet little is known about their genome content and function. Parasitoids that infect aphids are an important group with the potential for biological control. Their success depends on adapting to develop inside aphids and overcoming both host aphid defenses and their protective endosymbionts.<h4>Results</h4>We present the de novo genome assemblies, detailed annotation, and comparative ...[more]

PMID: 32471448

Similar Datasets

Project description:CD47 is a transmembrane glycoprotein that is ubiquitously expressed in different organs and tissues (Barclay and Van den Berg 2014; Liu, et al. 2017). In the human immune system, CD47 interacts with some integrins, two counter-receptor signal regulator protein (SIRP) family members, and the secreted thrombospondin-1 (TSP1) (Barclay and Van den Berg 2014; Gao, et al. 2016; Kaur, et al. 2013; Oldenborg, et al. 2000). CD47 has two established roles in the immune system. The CD47-SIRPα interaction was identified as a critical innate immune checkpoint, which delivers an antiphagocytic signal to macrophages and inhibits neutrophil cytotoxicity (Martínez- Sanz, et al. 2021). Its interaction with inhibitory SIRPα is a physiological anti-phagocytic “don’t eat me” signal on circulating red blood cells that is co-opted by cancer cells (Matlung, et al. 2017). Many malignant cells overexpress CD47 (Betancur, et al. 2017; Chao, et al. 2011; Jaiswal, et al. 2009; Majeti, et al. 2009; Oronsky, et al. 2020; Petrova, et al. 2017). CD47/SIRPα-targeted therapeutics have been developed to overcome this immune checkpoint for cancer treatment (Kaur, et al. 2020; Matlung, et al. 2017). Secondly, engagement of CD47 on T cells by TSP1 regulates their differentiation and survival (Grimbert, et al. 2006; Lamy, et al. 2007) and inhibits T cell receptor signaling and antigen presentation by dendritic cells (DCs) (Kaur, et al. 2014; Li, et al. 2002; Liu, et al. 2015; Miller, et al. 2013; Soto-Pantoja, et al. 2014; Weng, et al. 2014). TSP1/CD47 signaling has similar inhibitory functions to limit NK cell activation (Kim, et al. 2008; Nath, et al. 2018; Nath, et al. 2019; Schwartz, et al. 2019) and IL1β production by macrophages (Stein, et al. 2016). CD47 is therefore a checkpoint that regulates both innate and adaptive immunity. The recent understanding of CD47 antagonism associated with increased antigen presentation by DCs (Liu, et al. 2016) and natural killer cell cytotoxicity (Nath, et al. 2019) contributes to the heightened interest in CD47 as a therapeutic target (Kaur, et al. 2020).