Proteomics

Dataset Information

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First Global Proteomic Analysis of Lysine Malonylation in Toxoplasma gondii


ABSTRACT: Lysine malonylation (Kmal) is a new posttranslational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite Toxoplasma gondii is limited. In this study, we performed the first global profiling of malonylated proteins in T. gondii tachyzoites using affinity enrichment and LC-MS/MS analysis. Three experiments performed in tandem revealed 331, 367, 373 Kmal sites on 227, 252, 241 malonylated proteins, respectively. Computational analysis showed the identified malonylated proteins to be localized in various subcellular compartments and involved in many cellular functions, particularly mitochondrial function and oxidation of fatty acids. Additionally, two conserved Kmal motifs with a strong bias for cysteine were detected. Taken together, these findings provide the first report of Kmal profile in T. gondii and should be an important resource for studying the physiological roles of Kmal in this parasite.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: Lan-Be Nie  

LAB HEAD: Xing-Quan Zhu

PROVIDER: PXD015809 | Pride | 2020-05-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
7464PIMa.zip Other
7464PIMa_1_exp1.raw Raw
7464PIMa_1_exp2.raw Raw
7464PIMa_1_exp3.raw Raw
7464PIMa_2_exp1.raw Raw
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Publications

Global Proteomic Analysis of Lysine Malonylation in <i>Toxoplasma gondii</i>.

Nie Lan-Bi LB   Liang Qin-Li QL   Du Rui R   Elsheikha Hany M HM   Han Nai-Jian NJ   Li Fa-Cai FC   Zhu Xing-Quan XQ  

Frontiers in microbiology 20200428


Lysine malonylation (Kmal) is a new post-translational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite <i>Toxoplasma gondii</i> is limited. In this study, we performed the first global profiling of malonylated proteins in <i>T. gondii</i> tachyzoites using affinity enrichment and Liquid chromatography-tan  ...[more]

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