Proteomics

Dataset Information

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Upstream ORF-encoded ASDURF is a novel prefoldin-like subunit of the PAQosome

ABSTRACT: The PAQosome is an eleven-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5’ UTR of ASNSD1 mRNA, encodes the twelfth subunit of the PAQosome. ASDURF, displays significant structural homology to beta-prefoldins and assembles with the five known subunits of the prefoldin-like module of the PAQosome to form a heterohexameric prefoldin-like complex. A model of the PAQosome prefoldin-like module is presented. The data presented here provides an example of ana eukaryotic uORF-encoded polypeptide whose function is not limited to cis-acting translational regulation of downstream coding sequence and highlights the importance of including alternative ORF products in proteomic studies.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Christian Poitras  

LAB HEAD: Benoit Coulombe

PROVIDER: PXD015848 | Pride | 2019-11-25

REPOSITORIES: Pride

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OF_20181214_COU_01.raw Raw
OF_20181214_COU_02.raw Raw
OF_20181214_COU_03.raw Raw
OF_20181214_COU_10.raw Raw
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Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome.

Cloutier Philippe P   Poitras Christian C   Faubert Denis D   Bouchard Annie A   Blanchette Mathieu M   Gauthier Marie-Soleil MS   Coulombe Benoit B  

Journal of proteome research 20191125 1

The PAQosome is an 11-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5' UTR of ASNSD1 mRNA, encodes the 12th subunit of the PAQosome. ASDURF displays significant structural homology to β-prefoldins and assembles with the five known subunits of the prefoldin-like module of the PAQosome to form a heterohexameric prefoldin-like complex. A model of the PAQosome prefoldin-like module  ...[more]

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