Dataset Information

Proteomic analysis of the human milieu of gamete /embryo maternal interaction

ABSTRACT: The improvement of the embryo culture media is of high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes and potential effects in the adult life. The ideal conditions for embryo development are those naturally occurring in the female reproductive tract, i.e., the oviductal and uterine fluids. In order to shed light on the differences between chemical and natural media, we performed the first comparative study of the low abundance proteins in plasma, uterine and oviductal fluid collected, simultaneously, from healthy and fertile women that underwent a salpingectomy. The rationale for this design derives from the fact that high-abundant proteins in these fluids are usually those coming from blood serum and frequently mask the detection of low abundant proteins with a potential significant role on specific processes related to the embryo-maternal interaction. The proteomic analysis by 1D-nano LC ESI-MSMS detected several proteins in higher amounts in oviductal fluid when compared to uterine and plasma samples (RL3, GSTA1, EZRI, DPYSL3, GARS, HSP90A). Such oviductal fluid proteins could be a target to improve fertilization rates and early embryo development, if used in the culture media. In conclusion, this study presents a high-throughput analysis of female reproductive tract fluids and contributes to the knowledge of oviductal and uterine secretome.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Uterine Fluid, Blood Plasma, Oviductal Fluid

SUBMITTER: Alberto Paradela

LAB HEAD: Pilar Coy

PROVIDER: PXD015980 | Pride | 2019-10-29

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20180704_Analuce_BRA_52FO.mgf	Mgf
	20180704_Analuce_BRA_52FO.wiff	Wiff
	20180704_Analuce_BRA_52FO.wiff.scan	Wiff
	20180704_Analuce_BRA_52FU.mgf	Mgf
	20180704_Analuce_BRA_52FU.wiff	Wiff

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Publications

Which Low-Abundance Proteins are Present in the Human Milieu of Gamete/Embryo Maternal Interaction?

Canha-Gouveia Analuce A Paradela A A Ramos-Fernández António A Prieto-Sánchez Maria Teresa MT Sánchez-Ferrer Maria Luisa ML Corrales Fernando F Coy Pilar P

International journal of molecular sciences 20191024 21

The improvement of the embryo culture media is of high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes, and potential effects in adult life. The ideal conditions for embryo development are those naturally occurring in the female reproductive tract, i.e., the oviductal and uterine fluids. To shed light on the differences between chemical and natural media, we performed the first comparative study of the low abundance proteins in plasma, uterine, and o ...[more]

PMID: 31653120

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