Proteomics

Dataset Information

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Mass spectrometry proteomics detecting interaction proteins of Kindlin-2


ABSTRACT: We aim to find out the Kindlin-2 interaction proteins. We transfected plasmids Flag or Flag-Kindlin-2 to HKC cell lines and then used Flag-M2 beads to do the coimmunoprecipitation experiments and followed by NuPAGE Tris-Acetate Mini Gels detection and stained by Coomassie brilliant blue. Compared the two groups,and then we will find the proteins interact with Kindlin-2.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Jiagui Song  

LAB HEAD: Hongquan Zhang

PROVIDER: PXD016008 | Pride | 2019-12-16

REPOSITORIES: Pride

Dataset's files

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MS170526-CON.raw Raw
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Publications

Kindlin-2 Inhibits the Hippo Signaling Pathway by Promoting Degradation of MOB1.

Song Jiagui J   Wang Tianzhuo T   Chi Xiaochun X   Wei Xiaofan X   Xu Sidi S   Yu Miao M   He Huiying H   Ma Ji J   Li Xueying X   Du Juan J   Sun Xiaoran X   Wang Yunling Y   Zhan Jun J   Zhang Hongquan H  

Cell reports 20191201 11


The Hippo signaling pathway plays a key role in development and cancer progression. However, molecules that intrinsically inhibit this pathway are less well known. Here, we report that the focal adhesion molecule Kindlin-2 inhibits Hippo signaling by interacting with and degrading MOB1 and promoting the interaction between MOB1 and the E3 ligase praja2. Kindlin-2 thus inhibits the phosphorylation of LATS1 and YAP and promotes YAP translocation into the nucleus, where it activates downstream Hipp  ...[more]

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