Dataset Information

Quantification of exercise-regulated ubiquitin-signalling

ABSTRACT: The maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute post-translational regulation and proteome remodelling during exercise. Here, we used quantitative proteomics to study ubiquitin-signalling dynamics in human skeletal muscle biopsies from healthy males before, during and after a single bout of high-intensity exercise. Exercise resulted in a marked depletion of protein ubiquitylation consistent with proteasome activation. This was also associated with acute post-translational modification of protein abundance via a network of proteases.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skeletal Muscle

SUBMITTER: Benjamin Parker

LAB HEAD: Benjamin Parker

PROVIDER: PXD016158 | Pride | 2020-03-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	160226_Fusion_BP_HuPostEx1_UbiQ_.rar	Other
	160226_Fusion_BP_HuPostEx1_UbiQ_1.raw	Raw
	160226_Fusion_BP_HuPostEx1_UbiQ_10.raw	Raw
	160226_Fusion_BP_HuPostEx1_UbiQ_11.raw	Raw
	160226_Fusion_BP_HuPostEx1_UbiQ_12.raw	Raw

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Publications

Quantification of exercise-regulated ubiquitin signaling in human skeletal muscle identifies protein modification cross talk via NEDDylation.

Parker Benjamin L BL Kiens Bente B Wojtaszewski Jørgen F P JFP Richter Erik A EA James David E DE

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20200305 4

The maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute posttranslational regulation and proteome remodeling during and after high-intensity exercise. Here, we used quantitative proteomics to study ubiquitin signaling dynamics in human skeletal muscle biopsies from healthy males before, during, and after a si ...[more]

PMID: 32141134

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