Dataset Information

Proteomics of saliva, plasma, and salivary gland tissue in Sjögren’s syndrome and non-Sjögren patients identify novel biomarker candidates

ABSTRACT: Objective: The aim of this study was to characterize and compare the proteome in whole saliva, plasma, and salivary gland tissue in patients with primary Sjögren’s syndrome (pSS) and patients having symptoms of pSS, but not fulfilling the classification criteria, and to search for diagnostic biomarker candidates for pSS. Methods: Liquid chromatography tandem mass spectrometry was conducted on whole saliva, plasma, and labial salivary gland tissue samples from 24 patients with pSS and 16 non-Sjögren control subjects (non-pSS). Gene Ontology (GO)-terms and Kyoto Encyclopedia of Genes and Genomes (KEGG)-pathways were applied for functional annotation. Results: 1,013 proteins were identified in whole saliva, 219 in plasma, and 3,166 in salivary gland tissue. In saliva, 40 proteins differed significantly between the two groups. In pSS, proteins involved in immunoinflammatory processes were upregulated, whereas proteins related to salivary secretion were downregulated. The combination of neutrophil elastase, calreticulin, and tripartite motif-containing protein 29 yielded a receiver-operating characteristic (ROC) value of 0.97 (CI 0.93-1.00). Protein expression in plasma and salivary gland tissue did not differ between the patient groups. Conclusion: The salivary proteome of patients with pSS differed from that of non-pSS patients, indicating that saliva proteomics represents a promising non-invasive diagnostic tool for pSS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Salivary Gland, Saliva

SUBMITTER: Maria Sembler-Møller

LAB HEAD: Maria Lynn Sembler-Møller

PROVIDER: PXD016231 | Pride | 2020-07-09

REPOSITORIES: Pride

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			Action	DRS
	20181205_QE5_nLC7_RJC_WIMS_DM_ss002_01.raw	Raw
	20181205_QE5_nLC7_RJC_WIMS_DM_ss004_01.raw	Raw
	20181205_QE5_nLC7_RJC_WIMS_DM_ss005_01.raw	Raw
	20181205_QE5_nLC7_RJC_WIMS_DM_ss006_01.raw	Raw
	20181205_QE5_nLC7_RJC_WIMS_DM_ss008_01.raw	Raw

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Proteomics of saliva, plasma, and salivary gland tissue in Sjögren's syndrome and non-Sjögren patients identify novel biomarker candidates.

Sembler-Møller Maria L ML Belstrøm Daniel D Locht Henning H Pedersen Anne Marie L AML

Journal of proteomics 20200612

<h4>Objective</h4>The aim of this study was to characterize and compare the proteome in whole saliva, plasma, and salivary gland tissue in patients with primary Sjögren's syndrome (pSS) and patients having symptoms of pSS, but not fulfilling the classification criteria, and to search for diagnostic biomarker candidates for pSS.<h4>Methods</h4>Liquid chromatography tandem mass spectrometry was conducted on whole saliva, plasma, and labial salivary gland tissue samples from 24 patients with pSS an ...[more]

PMID: 32540407

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