Dataset Information

Proteomic analysis of salivary glands of female Aedes aegypti Linn

ABSTRACT: Aedes aegypti is a major vector for dengue, chikungunya and yellow fever. Though salivary gland plays a crucial role in transmission of virus and vector life cycle, there is no global and unbiased published report on salivary gland proteome of Ae. aegypti. In this study, we have carried out mass spectrometry based proteomic analysis of salivary gland from adult female Ae. aegypti mosquitoes. By fractionating the proteins isolated from salivary gland on SDS-PAGE and analyzing the in-gel digested bands on high resolution mass spectrometry, we identified 1,205 proteins. This is by far the largest catalogue of salivary gland proteome of Ae. aegypti. These proteins were then assigned molecular functions and biological processes. Several immunity related pathways were found to be enriched in salivary gland. In addition, subset of proteins was predicted to secretory in nature and may play an important role during blood feeding. This study provides a useful resource of proteins expressed in salivary gland of Ae. aegypti female mosquitoes and will aid in biomedical research focused on development of transmission blocking vaccine.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Aedes Aegypti Aegypti

TISSUE(S): Salivary Gland Cell, Salivary Gland

DISEASE(S): Disease Free

SUBMITTER: Rakhi Dhawan

LAB HEAD: Ashwani Kumar

PROVIDER: PXD002468 | Pride | 2017-02-28

REPOSITORIES: Pride

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