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FACS sorted podocytes, mesangial cells

ABSTRACT: FACS sorted glomerular kidney cells from a single mouse were subjected to proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD016237 | Pride | 2022-10-13

REPOSITORIES: Pride

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			Action	DRS
	20190116_Q2_UC_ColID_752_ProjID_1556_Benzing_01.raw	Raw
	20190116_Q2_UC_ColID_752_ProjID_1556_Benzing_02.raw	Raw
	20190116_Q2_UC_ColID_752_ProjID_1556_Benzing_03.raw	Raw
	20190116_Q2_UC_ColID_752_ProjID_1556_Benzing_04.raw	Raw
	20190116_Q2_UC_ColID_752_ProjID_1556_Benzing_05.raw	Raw

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Publications

Tripartite Separation of Glomerular Cell Types and Proteomes from Reporter-Free Mice.

Hatje Favian A FA Wedekind Uta U Sachs Wiebke W Loreth Desiree D Loreth Desiree D Reichelt Julia J Demir Fatih F Kosub Christopher C Heintz Lukas L Tomas Nicola M NM Huber Tobias B TB Skuza Sinah S Sachs Marlies M Zielinski Stephanie S Rinschen Markus M MM Meyer-Schwesinger Catherine C

Journal of the American Society of Nephrology : JASN 20210601 9

<h4>Background</h4>The glomerulus comprises podocytes, mesangial cells, and endothelial cells, which jointly determine glomerular filtration. Understanding this intricate functional unit beyond the transcriptome requires bulk isolation of these cell types for biochemical investigations. We developed a globally applicable tripartite isolation method for murine mesangial and endothelial cells and podocytes (timMEP).<h4>Methods</h4>We separated glomerular cell types from wild-type or mT/mG mice <i> ...[more]

PMID: 34074698

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