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Cell wall proteome of wheat developing grain

ABSTRACT: The aim of this project is to highlight cell wall proteome of wheat developing at a key stage of its development (=250 GDD, start of grain filling with storage compounds). It's the first study in which endosperm were separated of outer layers in order to gain more information about the mechanisms of cell wall assembly and remodeling.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell

SUBMITTER: Mehdi CHERKAOUI

LAB HEAD: Fabienne GUILLON

PROVIDER: PXD010367 | Pride | 2018-10-16

REPOSITORIES: Pride

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			Action	DRS
	OB09509-filtered.pride.mgf.gz	Mgf
	OB09509-filtered.pride.mztab.gz	Mztab
	OB09509-filtered.xml.gz	Xml
	OB09509.mzXML	Mzxml
	OB09510-filtered.pride.mgf.gz	Mgf

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Publications

Cell Wall Proteome Investigation of Bread Wheat (Triticum Aestivum) Developing Grain in Endosperm and Outer Layers.

Cherkaoui Mehdi M Geairon Audrey A Lollier Virginie V Clemente Hélène San HS Larré Colette C Rogniaux Hélène H Jamet Elisabeth E Guillon Fabienne F Francin-Allami Mathilde M

Proteomics 20181025 23

The remodeling of cell wall polysaccharides is controlled by cell wall proteins (CWPs) during the development of wheat grain. This work describes for the first time the cell wall proteomes of the endosperm and outer layers of the wheat developing grain, which have distinct physiological functions and technological uses. Altogether 636 nonredundant predicted CWPs are identified with 337 proteins in the endosperm and 594 proteins in the outer layers, among which 295 proteins are present in both ti ...[more]

PMID: 30288912

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Wheat grain cell wall enriched proteome

Project description:The aim of this project is to highlight cell wall proteome of wheat developing at two key stage of its development (150 GDD : middle of endosperm cellularisation and 250 GDD : start of grain filling with storage compounds). It's the first study of wheat grain cell wall proteome in which endosperm were separated of outer layers in order to gain more information about the mechanisms of cell wall assembly and remodeling.

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impact of Cd-exposure on medicago sativa cell wall

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Transcription profiling of rice stigma

Project description:In angiosperms, stigma provides initial nutrients and guidance cues for pollen grain germination and tube growth. However, little is known about genes that regulate these processes in rice. Here we generate rice stigma-specific gene expression profiles through comparing genome-wide expression patterns of hand dissected unpollinated stigma at anthesis with seven tissues including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds of five days after pollination, 10-day-old embryo, 10-day-old endosperm as well as suspension cultured cells by using 57K Affymetrix rice whole genome array. In total, we identified 665 probe sets (550 genes) to be expressed specifically or predominantly in the stigma papillar cells of rice. Real-Time quantitative RT-PCR analysis of 34 selected genes confirmed their stigma-specific expression. The expression of five selected genes was further validated by RNA in situ hybridization. Gene annotation shows that several auxin-signaling components, transporters and stress-related genes are significantly overrepresented in the rice stigma gene set. We also found that genes involved in cell wall metabolism and cellular communication appear to be conserved in the stigma between rice and Arabidopsis. Our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and Arabidopsis. Experiment Overall Design: We generate rice stigma-specific gene expression profiles through comparing genome-wide expression patterns of hand dissected unpollinated stigma at anthesis with seven tissues including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds of five days after pollination, 10-day-old embryo, 10-day-old endosperm as well as suspension cultured cells by using 57K Affymetrix rice whole genome array.

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Analysis of the protein distribution within the wheat endosperm

Project description:Starchy endosperm proteins determine wheat quality and exhibit, besides a quantitative protein gradient, a qualitative protein gradient from the outer to inner starchy endosperm. The goal was to investigate the relative differences in protein composition between the aleurone, sub-aleurone and inner endosperm. Using laser microdissection followed by nanoLC-MS/MS, an innovative method combining a high spatial specificity and analytical selectivity in sample-limited situations, 780 proteins were detected and classified by function. Relatively more gluten proteins were detected in the sub-aleurone compared to inner endosperm. Gluten composition-wise, the sub-aleurone is relatively more enriched in ω-gliadins, but impoverished in LMW-GS and γ-gliadins. While a basic set of albumins and globulins is detected across the entire endosperm, some proteins, like puroindoline-B, display an increasing (or decreasing) gradient. Histological origin and relative positioning of the endosperm cells are hypothesized to drive the protein gradient. Knowledge on this gradient provides major opportunities for the wheat manufacturing industry.

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Dynamic and Parent-of-Origin Expression of Small RNAs in the Developing Maize Endosperm

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