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The STRIPAK dependent phosphorylation of distinct residues of GUL1 controls hyphal morphology and sexual fertility

ABSTRACT: STRIPAK is a highly conserved eukaryotic signalling complex controlling a wide range of developmental processes in microorganisms as well as humans. This study uses iTRAQ-based proteomic and phosphoproteomic analyses to expand the identification of differentially phosphorylated proteins, comparing the wildtype proteome of the model fungus S. macorspora to those from three STRIPAK single- and double deletion mutants. Among the differentially phosphorylated proteins, we detected Gul1, a homolog of GUL-1 from N. crassa and SSD1 from yeast. In this study, we further provide a functional analysis of Gul1, showing it controls sexual development and hyphal morphology in a phosphorylation dependent manner.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sordaria Macrospora

SUBMITTER: Bernhard Blank-Landeshammer

LAB HEAD: Albert Sickmann

PROVIDER: PXD016296 | Pride | 2020-09-30

REPOSITORIES: pride

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			Action	DRS
	BB_Sordaria2_iTRAQ_pH8.msf	Msf
	QExactiveHF02_03555.raw	Raw
	QExactiveHF02_03556.raw	Raw
	QExactiveHF02_03557.raw	Raw
	QExactiveHF02_03558.raw	Raw

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Project description:STRIPAK is a highly conserved eukaryotic signalling complex controlling a wide range of developmental processes in microorganisms as well as humans. This study uses iTRAQ-based proteomic and phosphoproteomic analyses to expand the identification of differentially phosphorylated proteins, comparing the wildtype proteome of the model fungus S. macorspora to those from three STRIPAK single- and double deletion mutants. Among the differentially phosphorylated proteins, we detected Gul1, a homolog of GUL-1 from N. crassa and SSD1 from yeast. In this study, we further provide a functional analysis of Gul1, showing it controls sexual development and hyphal morphology in a phosphorylation dependent manner.

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