Proteomics

Dataset Information

0

Wnt Regulation: Exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex

ABSTRACT: Wnt signaling plays key roles in development and disease, by regulating the stability of its key effector βcat. In the absence of Wnt signals, βcat is phosphorylated by the Wnt-regulatory destruction complex, ubiquitinated by an SCF-class E3 ubiquitin ligase, and destroyed by the proteasome. Binding of Wnt ligands to their Frizzled/LRP receptors stabilizes βcat, via the cytoplasmic effector Dsh. Here we explore two important questions in the field: Is there a direct transfer of βcat from the destruction complex to the E3 ligase, and how does Dsh interaction with the destruction complex protein Axin regulate destruction complex function?

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Kidney

SUBMITTER: Dennis Goldfarb  

LAB HEAD: Mark Peifer

PROVIDER: PXD016314 | Pride | 2020-04-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
PE012_APC_Total_2nd_Mira.raw Raw
txt.zip Other
Items per page:
1 - 2 of 2
altmetric image

Publications

Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex.

Schaefer Kristina N KN   Pronobis Mira I MI   Williams Clara E CE   Zhang Shiping S   Bauer Lauren L   Goldfarb Dennis D   Yan Feng F   Major M Ben MB   Peifer Mark M  

Molecular biology of the cell 20200304 10

Wnt signaling plays key roles in embryonic development and adult stem cell homeostasis and is altered in human cancer. Signaling is turned on and off by regulating stability of the effector β-catenin (β-cat). The multiprotein destruction complex binds and phosphorylates β-cat and transfers it to the SCF-TrCP E3-ubiquitin ligase for ubiquitination and destruction. Wnt signals act though Dishevelled to turn down the destruction complex, stabilizing β-cat. Recent work clarified underlying mechanism  ...[more]

Similar Datasets

Proteomics Dysregulation of Wnt/β-Catenin Signaling pathway via RNF146 upregulation in the prefrontal cortex is responsible for social deficit in a VPA-induced ASD mouse model
2023-07-24 | PXD036413 | Pride
Proteomics Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradationDisease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation
2022-10-14 | PXD031683 | Pride
Proteomics Identification of the E3 ligase(s) recruited by 955 to degrade CDK9
2024-05-24 | PXD039401 | Pride
Proteomics USP7 is a tumor-specific WNT activator for APC-mutated colorectal cancer by mediating B-catenin deu
2017-11-09 | PXD007947 | Pride
Transcriptomics Targeting Non-proteolytic Protein Ubiquitination for the Treatment of Diffuse Large B Cell Lymphoma (Affy SNP6.0 Arrays)
2016-09-01 | E-GEOD-73639 | biostudies-arrayexpress
Transcriptomics Targeting Non-proteolytic Protein Ubiquitination for the Treatment of Diffuse Large B Cell Lymphoma (Agilent Arrays)
2016-09-01 | E-GEOD-73281 | biostudies-arrayexpress
Transcriptomics Piperlongumine (PL) conjugates induce targeted protein degradation
2022-06-26 | GSE206612 | GEO
Proteomics Identification and Characterization of MCM3 as a KEAP1 Substrate
2017-10-12 | PXD003929 | Pride
Transcriptomics Effect of depletion of HOIP on gene expression during B16-F10 melanoma tumor development in immunedeficinet mice.
2024-04-01 | GSE206472 | GEO
Transcriptomics Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation
2022-03-30 | GSE197240 | GEO