Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Galectins expression profile in mice intestine - Galectin 3 interacting partners identification

ABSTRACT: In this present project, we aimed to physiologically dissect the contribution of glycolipid- and lectin-driven endocytosis mechanism that operate within the mice intestine. We therefore decided to first characterize the expression profile of galectins in this tissue and then to focus on the specific implication of galectin 3 in that context and identify a potential binding partner, that may use this endocytic process for its internalization.

INSTRUMENT(S): Q Exactive HF-X, Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Enterocyte

SUBMITTER: Valentin SABATET

LAB HEAD: Damarys Loew

PROVIDER: PXD016499 | Pride | 2020-12-07

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	C8445FD.msf	Msf
	C8445FD.raw	Raw
	Design.xls	Xls
	H0055BL.msf	Msf
	H0055BL.raw	Raw

Items per page:

1 - 5 of 15

Similar Datasets

Consequences of SF3B1 mutation on the proteome

Project description:In this study, we evaluated the differential proteome of SF3B1-mutated and SF3B1-Wild-Type uveal melanoma cells (isogenic Mel202 cells). We performed a quantitative label-free tandem liquid chromatography mass spectrometry (LC-MS/MS) analysis of SF3B1WT and SF3B1mut Mel202 cells.

2022-03-15 | PXD022726 | Pride

The role of early chromatin changes in X chromosome inactivation

Project description:During development, transcriptional and chromatin modification changes co-occur but the order and causality of events often remain unclear. We explore the interrelationship of these processes using the paradigm of X-chromosome inactivation (XCI). We initiate XCI in female, mouse embryonic stem cells by inducing Xist expression and monitor changes in transcription and chromatin by allele-specific TT-seq and ChIP-seq respectively. An unprecedented temporal resolution enabled identification of the earliest chromatin alterations during XCI. We demonstrate that HDAC3 interacts with both NCOR1 and NCOR2 and is pre-bound on the X chromosome where it deacetylates histones to promote efficient gene silencing. We also reveal the choreography of polycomb accumulation following Xist RNA coating, with PRC1-associated H2AK119Ub preceding PRC2-associated H3K27me3. Furthermore, polycomb-associated marks accumulate initially at large, intergenic domains and then spreads into genes but only in the context of gene silencing. Our results provide the hierarchy of chromatin events during XCI and demonstrate that some chromatin changes play key roles in mediating transcriptional silencing.

2019-01-17 | PXD011344 | Pride

The specific localization and function of Cdc42 isoforms

Project description:Cdc42 is a small GTPase protein whose role in polarity is widely studied. However, in vertebrates there exist 2 isoforms arising due to alternative splicing. The function and localization of these have not been studied with respect to cell polarity and cell migration. We therefore perform a mass spec screen to identify interactors of the isoforms to better understand their cellular functions.

2024-01-23 | PXD017477 | Pride

Potential interactors of alpha-tubulin acetyltransferase 1 (αTAT1/MEC17)

Project description:Cell adhesion to the extracellular matrix occurs through integrin-mediated focal adhesions, which sense the mechanical properties of the substrate and impact cellular functions such as cell migration. Mechanotransduction at focal adhesions affects the actomyosin network and contributes to cell migration. Despite being key players in cell adhesion and migration, the role of microtubules in mechanotransduction has been overlooked. Here, we show that substrate rigidity increases microtubule acetylation through β1 integrin signalling in primary rat astrocytes. Moreover, αTAT1, the enzyme responsible for microtubule acetylation, interacts with a major mechanosensing focal adhesion protein, Talin, and is able to tune the distribution of focal adhesions depending on the matrix rigidity. αTAT1 also reorganises the actomyosin network, increases traction force generation and cell migration speed on stiff substrates. Mechanistically, acetylation of microtubules promotes the release of microtubule-associated RhoGEF, GEF-H1 into the cytoplasm, which then leads to RhoA activation and high actomyosin contractility. Thus, we propose a novel feedback loop involving a crosstalk between microtubules and actin in mechanotransduction at focal adhesions whereby, cells sense the rigidity of the substrate through integrin-mediated adhesions, modulate their levels of microtubule acetylation, which then controls the actomyosin cytoskeleton and force transmission on the substrate to promote mechanosensitive cell migration.

2021-11-02 | PXD015871 | Pride

CD63/CD9-GFP extracellular vesicles LC-MS/MS

Project description:This study aimed to identify subpopulations of extracellular vesicles (EVs) secreted by HeLa cells, and determine markers which enable to identify them, and which indicate their endosomal or plasma membrane origin. We used CD63 and CD9 as markers of EVs, and followed their intracellular trafficking using the RUSH assay. We used mass spectrometry to identify specific or common proteins in immunoprecipitated GFP+ EVs from HeLa transfected with the RUSH CD63-GFP or CD9-GFP, after a short (3h) or a long (24h) trafficking time from the endoplasmic reticulum.

2021-05-17 | PXD021515 | Pride

CD44 regulates epigenetic plasticity by mediating iron endocytosis - part 1

Project description:The biological process termed Epithelial-to-Mesenchymal Transition (EMT) plays a central role in cancer cell invasion, metastasis, self-renewal and resistance to therapy. Here, we characterize using quantitative LC-MS/MS the global changes in proteins levels occurring during EMT induced by epidermal growth factor in breast cancer MDA-MB-468 cells.

2019-10-01 | PXD011447 | Pride

RagG interactome analysis in human breast MDA-MB-231 cells

Project description:RagC was immunoprecipitated from human breast cancer MDA-MB-231 cells treated or not with the Arp2/3 complex inhibitor, CK666, and the RagC interactome was analyzed by mass-spectrometry.

2023-09-04 | PXD027100 | Pride

Translation regulation mechanisms in astrocytes

Project description:Identifying the proteins associated with the polyribosomes in astrocytes from the mouse brain to unveil translation regulation mechanisms in these cells.

2023-07-20 | PXD033121 | Pride

Extracellular vesicles from fibroblasts stimulated by FGF2

Project description:We compared proteomic analysis of extracellular vesicles (EVs) secreted from human dermal fibroblasts, either control or stimulated by FGF2 and after immunoprecipitation with FGF2-coated beads to isolated FGF2-positive EVs.

2022-12-20 | PXD033141 | Pride

Rational Design of High Affinity Peptides Validates Histone Chaperone ASF1 as a New Epigenetic Target in Cancer

Project description:Starting from the structure of the human ASF1-histone complex, we developed a rational design strategy combining epitope tethering and optimization of interface contacts to identify a potent peptide inhibitor with a dissociation constant of 3 nM. LC-MS/MS was used to identify in vivo targets of the most prominent peptide inhibitor.

2019-09-25 | PXD013279 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data