Proteomics

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Proteomic adaptation of Streptococcus pneumoniae to the human antimicrobial peptide LL-37


ABSTRACT: Secreted antimicrobial peptides (AMPs) are an important part of the human innate immune system and prevent local and systemic infections by inhibiting bacterial growth in a concentration dependent manner. In the respiratory tract, the cationic peptide LL-37 is one of the most abundant AMPs and capable of building pore complexes in usually negatively charged bacterial membranes, leading to destruction of bacteria. However, adaptation mechanisms of several pathogens to LL-37 are already described and are known to weaken the antimicrobial effect of the AMP, for instance, by repulsion, export or degradation of the peptide. This study examines proteome wide changes in Streptococcus pneumoniae D39, the leading cause of bacterial pneumonia, in response to physiological concentrations of LL-37 by high resolution mass spectrometry. Our data indicate that pneumococci may use some of the known adaptation mechanisms to reduce the effect of LL-37 on their physiology, too. Additionally, several proteins seem to be involved in resistance to AMPs which have not been related to this process before, such as the teichoic acid flippase TacF (SPD_1128). Understanding colonization and infection relevant adaptations of the pneumococcus to AMPs, especially LL-37, could finally uncover new drug targets to weaken the burden of this widespread pathogen.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Streptococcus Pneumoniae Serotype 2 (strain D39 / Nctc 7466)

SUBMITTER: Pierre-Alexander Mücke  

LAB HEAD: Dörte Becher

PROVIDER: PXD016511 | Pride | 2020-03-19

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
190220_V2_PM3_PM_Rep_II_t2KS_01.raw Raw
190220_V2_PM3_PM_Rep_II_t2KS_02.raw Raw
190220_V2_PM3_PM_Rep_II_t2KS_03.raw Raw
190220_V2_PM3_PM_Rep_II_t2KS_04.raw Raw
190220_V2_PM3_PM_Rep_I_t2KS_01.raw Raw
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Publications

Proteomic Adaptation of <i>Streptococcus pneumoniae</i> to the Human Antimicrobial Peptide LL-37.

Mücke Pierre-Alexander PA   Maaß Sandra S   Kohler Thomas P TP   Hammerschmidt Sven S   Becher Dörte D  

Microorganisms 20200314 3


Secreted antimicrobial peptides (AMPs) are an important part of the human innate immune system and prevent local and systemic infections by inhibiting bacterial growth in a concentration-dependent manner. In the respiratory tract, the cationic peptide LL-37 is one of the most abundant AMPs and capable of building pore complexes in usually negatively charged bacterial membranes, leading to the destruction of bacteria. However, the adaptation mechanisms of several pathogens to LL-37 are already de  ...[more]

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