Dataset Information

Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to nonoptimal temperatures under anaerobic conditions- SWATH data

ABSTRACT: Elucidation of the mechanism of temperature tolerance in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable sound industrial processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- (12 ºC) and-supra (39 ºC) optimal temperatures under chemostat conditions, in which specific growth rate–dependent changes are eliminated. In contrast to previous studies, we employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains was performed by SWATH-MS, allowing the quantification of 997 proteins. Our analysis revealed that temperature response differs between the strains, highlighting the precision with which each strain responds to changes in its environment; however, we also found some common responsive proteins among the strains, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome than supra-optimal temperature. Further, the proteomic data evidenced that the cold response involves a strong repression of translation related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation. On the other hand, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects yeast proteome, which can be a step forward for research regarding the comprehension and the improvement of yeast thermotolerance.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Luz Valero

LAB HEAD: José Manuel Guillamón

PROVIDER: PXD016567 | Pride | 2021-01-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	FG_180420-LibPool-IonLibrary.txt	Txt
	FG_180420-LibPool.group	Other
	FG_180420-LibPool.mgf	Mgf
	FG_180424-Swath.1.wiff	Wiff
	FG_180424-Swath.1.wiff.scan	Wiff

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Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions.

Pinheiro Tânia T Lip Ka Ying Florence KYF García-Ríos Estéfani E Querol Amparo A Teixeira José J van Gulik Walter W Guillamón José Manuel JM Domingues Lucília L

Scientific reports 20201218 1

Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking ...[more]

PMID: 33339840

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