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Proteomics of psychrotolerant Trichococcus patagoniensis and characterization of the produced exopolysaccharides as inulin

ABSTRACT: The psychrotolerant Trichococcus patagoniensis was previously reported to produce exopolysaccharides (EPS) that covers the cells, when grown at a low temperature. Although, T. patagoniensis has an optimal growth at 30 ºC, it still can grow at -5 ºC. This capability of T. patagoniensis to grow at subzero temperatures is expected to be reflected in the proteome, membrane lipid composition and/or EPS production. This study aimed to get insight into the cold adaptation mechanisms of T. patagoniensis. We compared the proteomes of cells grown at 0 and 30 ºC and the EPS that is formed.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Trichococcus Patagoniensis

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD011268 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	NS1-32_MaxQuant_results.zip	Other
	NS10_30oC_3b.raw	Raw
	NS11_30oC_3c.raw	Raw
	NS12_30oC_3d.raw	Raw
	NS13_30oC_4a.raw	Raw

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Publications

Stimulating Effect of Trichococcus flocculiformis on a Coculture of Syntrophomonas wolfei and Methanospirillum hungatei.

Doloman Anna A Boeren Sjef S Miller Charles D CD Sousa Diana Z DZ

Applied and environmental microbiology 20220614 13

Syntrophic anaerobic consortia comprised of fatty acid-degrading bacteria and hydrogen/formate-scavenging methanogenic archaea are of central importance for balanced and resilient natural and manufactured ecosystems: anoxic sediments, soils, and wastewater treatment bioreactors. Previously published studies investigated interaction between the syntrophic bi-cultures, but little information is available on the influence of fermentative bacteria on syntrophic fatty acid oxidation, even though ferm ...[more]

PMID: 35699440

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Genomic Run On (GRO): determination of the nascent transcriptional rates in a spt16 mutant

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The Effect of an Exopolysaccharide Probiotic Molecule from Bacillus subtilis on gene expression from four types of Breast Cancer Cells

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