Human NEK10 knockout airway ALI Fe-IMAC-MSMS

ABSTRACT: We identified inactivating mutations in NEK10, a poorly characterized human protein kinase, in a novel human bronchiectasis syndrome. In order to understand effects of loss of its function on the airway phosphoproteome, NEK10 was CRISPR/Cas9 targeted in human airway air-liquid interface (ALI) cultures with 2 independent guides and assayed by iron-enrichment phosphoproteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Respiratory Epithelial Cell, Lung

DISEASE(S): Bronchiectasis

SUBMITTER: Raghu Chivukula  

LAB HEAD: David M. Sabatini

PROVIDER: PXD016600 | Pride | 2020-01-09

REPOSITORIES: Pride