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Human NEK10 knockout airway ALI Fe-IMAC-MSMS

ABSTRACT: We identified inactivating mutations in NEK10, a poorly characterized human protein kinase, in a novel human bronchiectasis syndrome. In order to understand effects of loss of its function on the airway phosphoproteome, NEK10 was CRISPR/Cas9 targeted in human airway air-liquid interface (ALI) cultures with 2 independent guides and assayed by iron-enrichment phosphoproteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Respiratory Epithelial Cell, Lung

DISEASE(S): Bronchiectasis

SUBMITTER: Raghu Chivukula

LAB HEAD: David M. Sabatini

PROVIDER: PXD016600 | Pride | 2020-01-09

REPOSITORIES: pride

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	190214_28334_AS_L.raw	Raw
	190214_28335_AS_L.raw	Raw
	190214_28336_AS_L.raw	Raw
	190214_28337_AS_L.raw	Raw
	190214_28338_AS_L.raw	Raw

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A human ciliopathy reveals essential functions for NEK10 in airway mucociliary clearance.

Chivukula Raghu R RR Montoro Daniel T DT Leung Hui Min HM Yang Jason J Shamseldin Hanan E HE Taylor Martin S MS Dougherty Gerard W GW Zariwala Maimoona A MA Carson Johnny J Daniels M Leigh Anne MLA Sears Patrick R PR Black Katharine E KE Hariri Lida P LP Almogarri Ibrahim I Frenkel Evgeni M EM Vinarsky Vladimir V Omran Heymut H Knowles Michael R MR Tearney Guillermo J GJ Alkuraya Fowzan S FS Sabatini David M DM

Nature medicine 20200120 2

Mucociliary clearance, the physiological process by which mammalian conducting airways expel pathogens and unwanted surface materials from the respiratory tract, depends on the coordinated function of multiple specialized cell types, including basal stem cells, mucus-secreting goblet cells, motile ciliated cells, cystic fibrosis transmembrane conductance regulator (CFTR)-rich ionocytes, and immune cells<sup>1,2</sup>. Bronchiectasis, a syndrome of pathological airway dilation associated with imp ...[more]

PMID: 31959991

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