Proteomics

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An interaction network of RNA-binding proteins involved in Drosophila oogenesis


ABSTRACT: During Drosophila oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and further validation in human cells allowed us to identify 26 previously unknown associations, besides recovering several well characterized interactions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Ovary

SUBMITTER: Nicolas Nalpas  

LAB HEAD: Fulvia Bono

PROVIDER: PXD016680 | Pride | 2020-06-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20161104_CO_0685FuBo_R01.raw Raw
20161104_CO_0685FuBo_R02.raw Raw
20161104_CO_0685FuBo_R03.raw Raw
20161104_CO_0685FuBo_R04.raw Raw
20161104_CO_0685FuBo_R05.raw Raw
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Publications

An Interaction Network of RNA-Binding Proteins Involved in <i>Drosophila</i> Oogenesis.

Bansal Prashali P   Madlung Johannes J   Schaaf Kristina K   Macek Boris B   Bono Fulvia F  

Molecular & cellular proteomics : MCP 20200617 9


During <i>Drosophila</i> oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole <i>Drosophila</i> ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and fu  ...[more]

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