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An interaction network of RNA-binding proteins involved in Drosophila oogenesis

ABSTRACT: During Drosophila oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and further validation in human cells allowed us to identify 26 previously unknown associations, besides recovering several well characterized interactions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Ovary

SUBMITTER: Nicolas Nalpas

LAB HEAD: Fulvia Bono

PROVIDER: PXD016680 | Pride | 2020-06-22

REPOSITORIES: Pride

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			Action	DRS
	20161104_CO_0685FuBo_R01.raw	Raw
	20161104_CO_0685FuBo_R02.raw	Raw
	20161104_CO_0685FuBo_R03.raw	Raw
	20161104_CO_0685FuBo_R04.raw	Raw
	20161104_CO_0685FuBo_R05.raw	Raw

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Publications

An Interaction Network of RNA-Binding Proteins Involved in Drosophila Oogenesis.

Bansal Prashali P Madlung Johannes J Schaaf Kristina K Macek Boris B Bono Fulvia F

Molecular & cellular proteomics : MCP 20200617 9

During Drosophila oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and fu ...[more]

PMID: 32554711

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