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Integrated of proteomics data refines key adaptation and regulatory processes during nitrogen starvation of Nannochloropsis oceanicaIntegrated of proteomics data refines key adaptation and regulatory processes during nitrogen starvation of Nannochloropsis oceanica

ABSTRACT: Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Nannochloropsis Oceanica Strain Imet1

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Wuxin You

LAB HEAD: Ansgar Poetsch

PROVIDER: PXD016699 | Pride | 2020-10-23

REPOSITORIES: Pride

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Integration of proteome and transcriptome refines key molecular processes underlying oil production in <i>Nannochloropsis oceanica</i>.

You Wuxin W Wei Li L Gong Yanhai Y Hajjami Mohamed El ME Xu Jian J Poetsch Ansgar A

Biotechnology for biofuels 20200618

<h4>Background</h4>Under nitrogen deficiency situation, <i>Nannochloropsis</i> spp. accumulate large amounts of lipids in the form of triacylglycerides (TAG). Mechanisms of this process from the perspective of transcriptome and metabolome have been obtained previously, yet proteome analysis is still sparse which hinders the analysis of dynamic adaption to nitrogen deficiency. Here, proteomes for 3 h, 6 h, 12 h, 24 h, 48 h and 10th day of nitrogen deplete (N-) and replete (N+) conditions were obt ...[more]

PMID: 32565907

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			Action	DRS
	MaxQuant_Output.zip	Other
	N-_12h_1-1.raw	Raw
	N-_12h_1-2.raw	Raw
	N-_12h_1-3.raw	Raw
	N-_12h_1-4.raw	Raw