Dataset Information

Comparison of the Human Neuronal Proteome upon Japanese Encephalitis or West Nile Virus Infection and potential role of mosquito saliva in neuropathogenesis

ABSTRACT: Neurotropic flavivirus Japanese encephalitis virus (JEV) and West Nile virus (WNV) are amongst the leading causes of encephalitis. Using label-free quantitative proteomics, we identified proteins differentially expressed upon JEV (gp-3, RP9) or WNV (IS98) infection of human neuroblastoma cells. Both viruses were associated with the up-regulation of immune response (IFIT1/3/5, ISG15, OAS, STAT1, IRF9) and the down-regulation of SSBP2, involved in gene expression, as well as PAM, involved in neuropeptide amidation. Proteins associated to membranes, involved in extracellular matrix organization and collagen metabolism represented major clusters down-regulated by JEV and WNV. Moreover, transcription regulation and mRNA processing clusters were also heavily regulated by both neurotropic flaviviruses. If the proteome of neuroblastoma cells infected by JEV or WNV was significantly modulated in the presence of mosquito saliva, both viruses showed distinct patterns. Mosquito saliva favored the modulation of proteins associated with gene regulation in JEV infected neuroblastoma cells while it was the modulation of proteins associated with protein maturation, signal transduction and ion transporters in the case of WNV infected neuroblastoma cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) West Nile Virus Culex Pipiens Pipiens Japanese Encephalitis Virus

TISSUE(S): Brain

SUBMITTER: MARIETTE MATONDO

LAB HEAD: Benoit Besson

PROVIDER: PXD016805 | Pride | 2020-05-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20141205_Q_TC_ColTC22_Choumet_JE_-EGS_2011.raw	Raw
	20141205_Q_TC_ColTC22_Choumet_JE_-EGS_2111.raw	Raw
	20141205_Q_TC_ColTC22_Choumet_JE_-EGS_2711.raw	Raw
	20141205_Q_TC_ColTC22_Choumet_JE_EGS_2011.raw	Raw
	20141205_Q_TC_ColTC22_Choumet_JE_EGS_2111.raw	Raw

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Publications

Comparison of a human neuronal model proteome upon Japanese encephalitis or West Nile Virus infection and potential role of mosquito saliva in neuropathogenesis.

Besson Benoit B Basset Justine J Gatellier Sandrine S Chabrolles Hélène H Chaze Thibault T Hourdel Véronique V Matondo Mariette M Pardigon Nathalie N Choumet Valérie V

PloS one 20200506 5

Neurotropic flavivirus Japanese encephalitis virus (JEV) and West Nile virus (WNV) are amongst the leading causes of encephalitis. Using label-free quantitative proteomics, we identified proteins differentially expressed upon JEV (gp-3, RP9) or WNV (IS98) infection of human neuroblastoma cells. Data are available via ProteomeXchange with identifier PXD016805. Both viruses were associated with the up-regulation of immune response (IFIT1/3/5, ISG15, OAS, STAT1, IRF9) and the down-regulation of SSB ...[more]

PMID: 32374750

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