Proteomics

Dataset Information

0

MS-based quantitative proteomic analysis of total proteomes from T. gondii strains upon MORC’s depletion and TgHDAC3 inhibition.

ABSTRACT: T. gondii has a complex life cycle typified by an asexual development taking place in vertebrate, and a sexual reproduction which occurs exclusively in felids and thereby is less studied. The developmental transitions rely on changes in gene expression patterns, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified T. gondii microrchidia (MORC) protein as an upstream transcriptional repressor of sexual commitment. To explore the molecular role of MORC, we conducted mass spectrometry-based quantitative proteomic analyses to monitor the proteome changes upon MORC’s depletion and TgHDAC3 inhibition.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: Yohann Couté  

LAB HEAD: Virginie Brun

PROVIDER: PXD016845 | Pride | 2020-01-09

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
QEx2_004005_.raw Raw
QEx2_004006.raw Raw
QEx2_004007.raw Raw
QEx2_004008.raw Raw
QEx2_004010.raw Raw
Items per page:
1 - 5 of 78
altmetric image

Publications

Toxoplasma gondii has a complex life cycle that is typified by asexual development that takes place in vertebrates, and sexual reproduction, which occurs exclusively in felids and is therefore less studied. The developmental transitions rely on changes in the patterns of gene expression, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified the T. gondii microrchidia (M  ...[more]

Similar Datasets

Proteomics Mass spectrometry-based characterization of Toxoplasma gondii MORC interactome
2020-01-09 | PXD016846 | Pride
Genomics A MORC-driven transcriptional switch controls Toxoplasma developmental trajectories and sexual commitment
2019-12-21 | GSE136060 | GEO
Transcriptomics A MORC-driven transcriptional switch controls Toxoplasma developmental trajectories and sexual commitment
2019-12-21 | GSE136123 | GEO
Transcriptomics AP2XII-1 and AP2XI-2 Suppress Schizogony Gene Expression in Toxoplasma gondii
2023-12-10 | GSE249604 | GEO
Proteomics In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming
2023-09-16 | PXD039400 | Pride
Proteomics Elucidating the diverse roles of acetyl-CoA in the cytosol and mitochondrion of Toxoplasma gondii
2020-05-07 | PXD016133 | Pride
Genomics To identify HDAC3 occupancy in the genomes of ME49 and ΔXII-5 strains
2024-12-19 | GSE283441 | GEO
Transcriptomics AP2XII-2-dependent gene regulation in Toxoplasma gondii tachyzoites
2023-01-24 | GSE217226 | GEO
Genomics AP2XII-2 binding sites in Toxoplasma gondii
2023-01-24 | GSE217220 | GEO
Proteomics Mass spectrometry-based proteomic characterization of T. gondii AP2XI-2 and AP2XII-1 interactomes
2023-09-16 | PXD042658 | Pride