Proteomics

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MS-based quantitative proteomic analysis of total proteomes from T. gondii strains upon MORC’s depletion and TgHDAC3 inhibition.

ABSTRACT: T. gondii has a complex life cycle typified by an asexual development taking place in vertebrate, and a sexual reproduction which occurs exclusively in felids and thereby is less studied. The developmental transitions rely on changes in gene expression patterns, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified T. gondii microrchidia (MORC) protein as an upstream transcriptional repressor of sexual commitment. To explore the molecular role of MORC, we conducted mass spectrometry-based quantitative proteomic analyses to monitor the proteome changes upon MORC’s depletion and TgHDAC3 inhibition.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: Yohann Couté  

LAB HEAD: Virginie Brun

PROVIDER: PXD016845 | Pride | 2020-01-09

REPOSITORIES: Pride

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Toxoplasma gondii has a complex life cycle that is typified by asexual development that takes place in vertebrates, and sexual reproduction, which occurs exclusively in felids and is therefore less studied. The developmental transitions rely on changes in the patterns of gene expression, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified the T. gondii microrchidia (M  ...[more]

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