Dataset Information

Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7

ABSTRACT: Clostridium sp. strain CT7 is a new emerging microbial cell factory with high butanol ratio owing to the non-traditional butanol fermentation mode with uncoupled acetone and 1,3-propanediol formation. Significant change of products profile was shown in glycerol- and glucose-fed strain CT7, especially much higher butanol and lower volatile fatty acids production from glycerol-fed one. However, the mechanism of this interesting phenomenon was still unclear. To better elaborate the bacterial response towards glycerol and glucose, the quantitative proteomic analysis through iTRAQ strategy was performed to reveal the regulated proteomic expression levels under different substrates. Proteomics data showed highly increased proteomic expression levels of proteins related with glycerol utilization and solvent generation under glycerol media. In addition, the up-regulation of hydrogenases, ferredoxins and electron-transferring proteins may attribute to the internal redox balance, while the earlier triggered sporulation response in glycerol-fed media may be associated with the higher butanol fermentation. This study will provide the platform for metabolic engineering of this emerging industrial microorganism for more efficient butanol production from glycerol.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Uncultured Clostridium Sp.

TISSUE(S): Whole Body

SUBMITTER: xiaonan fu

LAB HEAD: Fengxue Xin

PROVIDER: PXD016848 | Pride | 2021-09-08

REPOSITORIES: Pride

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Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7.

Jiang Yujia Y Wu Ruofan R Lu Jiasheng J Dong Weiliang W Zhou Jie J Zhang Wenming W Xin Fengxue F Jiang Min M

Microbial cell factories 20210109 1

Clostridium sp. strain CT7 is a new emerging microbial cell factory with high butanol production ratio owing to its non-traditional butanol fermentation mode with uncoupled acetone and 1,3-propanediol formation. Significant changes of metabolic products profile were shown in glycerol- and glucose-fed strain CT7, especially higher butanol and lower volatile fatty acids (VFAs) production occurred from glycerol-fed one. However, the mechanism of this interesting phenomenon was still unclear. To bet ...[more]

PMID: 33422075

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