Dataset Information

Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum

ABSTRACT: Blood contains hundreds of proteins, reflecting ongoing cellular processes and immune reactions. Angiostrongylus vasorum infection is associated with a perturbed blood protein profile in dogs. However, the literature currently available lacks the necessary depth of analysis in order to resolve the observed pathologies in A. vasorum infections, including bleeding disorders. Using sera from 8 experimentally-infected dogs (i) before infection with A. vasorum, (ii) 34 days post-infection (p.i.; immature infection), and (iii) 75 days p.i. (mature patent infection), serum proteins were measured using liquid chromatography, tandem mass spectrometry (LC-MS/MS). For 2 dogs, serum was analyzed at days 104 and 230 p.i. additionally. A data-independent acquisition workflow was employed in order to generate quantitative data. Following computational analysis, we identified 139 up- and down-regulated proteins following infection (log2 ratio cutoff ≥ 1.0; q-value ≤ 0.05). Differences in serum profiles were most pronounced at day 75 p.i. compared to before infection. Among up-regulated proteins, chitinase 3, several saposin-like proteins, and heat shock proteins were found greatly increased (log2 fold-changes ≥ 5). Levels of pulmonary surfactant protein B were elevated on day 34 p.i. already, in the prepatent phase. Pathway enrichment revealed that complement (especially the lectin pathway) and coagulation cascades as significantly affected upon analysis of down-regulated proteins. Among them were mannan-binding lectin serine peptidases, ficolin, and coagulation factors. These results reflect the ongoing immune response and stress imposed to the lungs by the parasite. In addition, they bring new elements towards understanding the coagulopathies observed in some A. vasorum-infected dogs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Angiostrongylus Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Serum

SUBMITTER: Tobias Kockmann

LAB HEAD: Manuela Schnyder

PROVIDER: PXD016881 | Pride | 2021-01-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170822_03_60696_1605_d_-7.raw	Raw
	20170822_04_60709_8028_d_75.raw	Raw
	20170822_05_60717_5074_d_34.raw	Raw
	20170822_06_60710_2200_d_-7.raw	Raw
	20170822_07_60707_8028_d_-7.raw	Raw

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Publications

Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum sheds light on the pathogenesis of canine angiostrongylosis.

Tritten Lucienne L Gillis-Germitsch Nina N Kockmann Tobias T Schnyder Manuela M

Scientific reports 20210111 1

Blood contains hundreds of proteins, reflecting ongoing cellular processes and immune reactions. Infections with the blood-dwelling cardiopulmonary nematode Angiostrongylus vasorum in dogs manifest with a broad spectrum of clinical signs including respiratory distress, bleeding diathesis and neurological signs, and are associated with a perturbed blood protein profile in dogs. However, current knowledge does not completely explain the observed pathologies induced by A. vasorum infections, includ ...[more]

PMID: 33431914

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