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Transcription profiling of rat whole lung during hantavirus infection to investigate sex specific differences

ABSTRACT: Gene expression profiles were examined in whole lung tissue collected from male and female Long-Evans rats at different time points after inoculation with Seoul virus (i.e., the species-specific hantavirus that infects Norway rats); We used microarray analyses to analyze global gene expression differences between the sexes during hantavirus infection Experiment Overall Design: Gonadally-intact male and female rats were inoculated with 10^4 pfu of Seoul virus (strain SR-11) and lung samples were collected 0, 3, 15, and 40 days post-inoculation (p.i.) to assess gene expression patterns during the acute (i.e., days 3-15 p.i.) and persistent (i.e., day 40 p.i.) phases of infection

ORGANISM(S): Rattus norvegicus

SUBMITTER: Sabra Lynn Klein

PROVIDER: E-GEOD-7271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sex differences in the recognition of and innate antiviral responses to Seoul virus in Norway rats.

Hannah Michele F MF Bajic Vladimir B VB Klein Sabra L SL

Brain, behavior, and immunity 20071128 4

Among rodents that carry hantaviruses, more males are infected than females. Male rats also have elevated copies of Seoul virus RNA and reduced transcription of immune-related genes in the lungs than females. To further characterize sex differences in antiviral defenses and whether these differences are mediated by gonadal hormones, we examined viral RNA in the lungs, virus shedding in saliva, and antiviral defenses among male and female rats that were intact, gonadectomized neonatally, or gonad ...[more]

PMID: 18053684

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