Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

45 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of rat whole lung during hantavirus infection to investigate sex specific differences

ABSTRACT: Gene expression profiles were examined in whole lung tissue collected from male and female Long-Evans rats at different time points after inoculation with Seoul virus (i.e., the species-specific hantavirus that infects Norway rats); We used microarray analyses to analyze global gene expression differences between the sexes during hantavirus infection Experiment Overall Design: Gonadally-intact male and female rats were inoculated with 10^4 pfu of Seoul virus (strain SR-11) and lung samples were collected 0, 3, 15, and 40 days post-inoculation (p.i.) to assess gene expression patterns during the acute (i.e., days 3-15 p.i.) and persistent (i.e., day 40 p.i.) phases of infection

ORGANISM(S): Rattus norvegicus

SUBMITTER: Sabra Lynn Klein

PROVIDER: E-GEOD-7271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Sex differences in the recognition of and innate antiviral responses to Seoul virus in Norway rats.

Hannah Michele F MF Bajic Vladimir B VB Klein Sabra L SL

Brain, behavior, and immunity 20071128 4

Among rodents that carry hantaviruses, more males are infected than females. Male rats also have elevated copies of Seoul virus RNA and reduced transcription of immune-related genes in the lungs than females. To further characterize sex differences in antiviral defenses and whether these differences are mediated by gonadal hormones, we examined viral RNA in the lungs, virus shedding in saliva, and antiviral defenses among male and female rats that were intact, gonadectomized neonatally, or gonad ...[more]

PMID: 18053684

Similar Datasets

Sex differences in gene expression profiles during hantavirus infection of rats

Project description:Gene expression profiles were examined in whole lung tissue collected from male and female Long-Evans rats at different time points after inoculation with Seoul virus (i.e., the species-specific hantavirus that infects Norway rats) We used microarray analyses to analyze global gene expression differences between the sexes during hantavirus infection Keywords: time course

2007-10-30 | GSE7271 | GEO

Sex differences in immune response

Project description:In the field, adult male rodents are more frequently infected with hantaviruses than females. Early data suggests that sex steroid hormones modulate sex differences in host immune response. This project focuses on elucidating sex differences in gene expression in the lungs of infected males 15 and 40 days post infection with Seoul virus (naturally occurring hantavirus in Norway rats) relative to infected females 15 and 40 days post infection on 12 RG_U34 GeneChips. Keywords: other

2003-07-16 | GSE488 | GEO

Transcription profiling of difference in immune response to hantavirus by Rattus norvegicus male and female animals

2007-10-10 | E-GEOD-488 | biostudies-arrayexpress

Gene expression profile changes in brains of VSV-infected wild-type and Ifit2 knockout mice at 6 days after infection

Project description:Vesicular stomatitis virus replicates with marked effciency in Ifit2 knockout mouse brains late after infection (6 days after intranasal inoculation), compared to wt brains. Changes in gene expression, especially of interferon-stimulated genes, between late in infection (at 6 d p.i.) compared to early during infection (2 d p.i.) were examined in comparison to wild-type.

2012-11-14 | GSE33678 | GEO

Gene expression profile changes in brains of VSV-infected wild-type and Ifit2 knockout mice at 6 days after infection

2012-11-14 | E-GEOD-33678 | biostudies-arrayexpress

Genome-wide expression of liver and udder tissue in response to E. coli treatment in early lactation dairy cows

Project description:We performed a global gene-expression analysis of mammary gland and liver tissue collected from dairy cows that had been exposed to a controlledÂ E. coliÂ infection. At time = 0, each of the periparturient dairy cows received 20-40 colony-forming units of live E. coli in one front quarter of the udder. Biopsy samples of healthy and infected udder tissue were collected at T = 24 h post-infection (p.i.) and at T = 192 h p.i. to represent the acute phase response (APR). A time series of liver biopsies was collected at -144, 12, 24, and 192 h relative to time of inoculation. Hf=right forward teat Vf=left forward teat

2016-07-01 | E-GEOD-75379 | biostudies-arrayexpress

Transcription profiling of potato genotypes hypersensitive vs non-hypersensitive to potato virus A upon infection with the virus

Project description:Potato genotypes from a diploid potato population were divided in two groups based on their response to Potato virus A (PVA). Plants exhibiting hypersensitive response were compared to plants exhibiting non-necrotic response (i.e. blocking virus movement without cell death).<br>The comparisons were made before inoculation and 12 and 24 hours post-inoculation.<br>

2010-09-30 | E-MEXP-2622 | biostudies-arrayexpress

Transcription profiling of Strongyloides ratti to host immunity

Project description:Strongyloides ratti is a parasitic nematode of rats and a laboratory model for nematode infection more generally. The response of two lines of S. ratti were compared in contrasting immunological environments: (i) day 5 post infection (p.i.) in naive rats; (ii) day 12 p.i. in naive rats; day 5 p.i. in rats previously immunised with 10 iL3s; and day 12 p.i. in rats previously immunised with 10 iL3s. The gene expression response of parasitic females were assayed using cDNA microarrays. Large numbers of responding genes were found (but with modest fold changes) and clusters of co-expressed genes identified with differences observed between worms taken from naive and previously exposed hosts and from the two time points.

2009-09-03 | E-MAXD-53 | biostudies-arrayexpress

Chikungunya virus phosphoproteomics

Project description:SILAC-labeled MRC-5 cells were seeded in 75-cm2 flasks 1 day before infection with CHIKV-LS3 [1] at MOI 5. One hour post infection (h p.i.), the inoculum was removed and replaced with SILAC DMEM containing 2% dialyzed FBS, 0.280 mM arginine, 0.384 mM lysine, 0.5 mM proline, 25 mM HEPES, 2 mM L-Glutamine and 1% NEAA. At 2, 8, and 12 h p.i., infected and mock-infected cells were harvested for phosphoproteomics analysis by lysis in 4% SDS, 0.1M Tris pH 7.6, followed by heating to 96°C for 10 min. At 12 h p.i,. protein lysates for western blot (WB) analysis were harvested in 4× Laemmli sample buffer (LSB) (100 mM Tris-HCl, pH 6.8, 40% glycerol, 8% SDS, 40 mM DTT, 0,04 mg/ml bromophenol blue) and cells grown on coverslips were fixed in 3% PFA in PBS . The experiment was performed in duplicate with a label swap.

2022-02-28 | PXD009381 | Pride

Transcription profiling of rat tracheal epithelial RTEC11 cells

Project description:We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell growth arrest and differentiation in tracheal epithelial RTEC11 cells harboring temperature-sensitive simian virus 40 large T-antigen by using an Affymetrix GeneChip system. Tracheal epithelial RTEC11 cells used in this study were derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen. Although the cells grew continuously at the permissive temperature, the nonpermissive temperature led to cell growth arrest and differentiation. Experiment Overall Design: A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at permissive (33Â°C) or nonpermissive (39Â°C) temperature for 3 days.

2008-06-16 | E-GEOD-8444 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data