Dataset Information

Identification of proteome changes in apoptotic NSCLC cells

ABSTRACT: Here, we used an unbiased, functional target-discovery platform to identify immunogenic proteins from primary non-small cell lung cancer (NSCLC) cells that had been induced to apoptosis by cisplatin (CDDP) treatment in vitro, as compared with their live counterparts. Among the multitude of proteins identified, some of them were represented as fragmented proteins in apoptotic tumor cells, and acted as non-mutated neoantigens. Only the fragmented proteins elicited effective multi-specific T cell responses, upon a chemotherapy protocol including CDDP. Importantly, these responses further increased significantly upon anti-PD-1 therapy, and correlated with patients’ survival and decreased PD-1 expression.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

SUBMITTER: Alessio Grimaldi

LAB HEAD: Vincenzo Barnaba

PROVIDER: PXD016997 | Pride | 2020-05-27

REPOSITORIES: Pride

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Combination of chemotherapy and PD-1 blockade induces T cell responses to tumor non-mutated neoantigens.

Grimaldi Alessio A Cammarata Ilenia I Martire Carmela C Focaccetti Chiara C Piconese Silvia S Buccilli Marta M Mancone Carmine C Buzzacchino Federica F Berrios Julio Rodrigo Giron JRG D'Alessandris Nicoletta N Tomao Silverio S Giangaspero Felice F Paroli Marino M Caccavale Rosalba R Spinelli Gian Paolo GP Girelli Gabriella G Peruzzi Giovanna G Nisticò Paola P Spada Sheila S Panetta Mariangela M Letizia Cecere Fabiana F Visca Paolo P Facciolo Francesco F Longo Flavia F Barnaba Vincenzo V

Communications biology 20200225 1

Here, we developed an unbiased, functional target-discovery platform to identify immunogenic proteins from primary non-small cell lung cancer (NSCLC) cells that had been induced to apoptosis by cisplatin (CDDP) treatment in vitro, as compared with their live counterparts. Among the multitude of proteins identified, some of them were represented as fragmented proteins in apoptotic tumor cells, and acted as non-mutated neoantigens (NM-neoAgs). Indeed, only the fragmented proteins elicited effectiv ...[more]

PMID: 32099064

			Action	DRS
	SILAC1PEAK.zip	Other
	SILAC1RAW.zip	Other
	SILAC1SEARCH.zip	Other
	SILAC2PEAK.zip	Other
	SILAC2RAW.zip	Other

Dataset Information

Identification of proteome changes in apoptotic NSCLC cells

Dataset's files

Publications

Combination of chemotherapy and PD-1 blockade induces T cell responses to tumor non-mutated neoantigens.

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