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Excess copper-induced alterations of protein profiles by a 2-DE based MS method and related physiological parameters in Citrus leaves

ABSTRACT: After long-term Copper-toxicity treatment,2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis and Citrus grandis leaves samples.

INSTRUMENT(S): TripleTOF 5600, MALDI Synapt MS

ORGANISM(S): Citrus Sinensis X Citrus Reticulata

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Lin-Tong Yang

LAB HEAD: Lin-Tong Yang

PROVIDER: PXD017049 | Pride | 2020-05-27

REPOSITORIES: Pride

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			Action	DRS
	DAT.rar	Other
	G1-F003863.dat	Other
	G10-F003897.dat	Other
	G11-F003872.dat	Other
	G12-F003873.dat	Other

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Publications

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves.

Huang Wei-Lin WL Wu Feng-Lin FL Huang Hui-Yu HY Huang Wei-Tao WT Deng Chong-Ling CL Yang Lin-Tong LT Huang Zeng-Rong ZR Chen Li-Song LS

Plants (Basel, Switzerland) 20200228 3

This present study examined excess copper (Cu) effects on seedling growth, leaf Cu concentration, gas exchange, and protein profiles identified by a two-dimensional electrophoresis (2-DE) based mass spectrometry (MS) approach after Citrus sinensis and Citrus grandis seedlings were treated for six months with 0.5 (control), 200, 300, or 400 μM CuCl2. Forty-one and 37 differentially abundant protein (DAP) spots were identified in Cu-treated C. grand ...[more]

PMID: 32121140

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Project description:After long-term low pH treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis and Citrus grandis leaves samples.

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Proteome profile analysis of boron-induced alleviation of aluminum-toxicity in Citrus grandis roots by 2-dimentional electrophoresis (2-DE) technique

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Phosphorus-mediated alleviation of aluminum toxicity revealed by iTRAQ technique in Citrus grandis roots

Project description:Citrus grandis seedlings were irrigated with nutrient solutions containing two levels of aluminium (Al; 0 mM and 1.2 mM AlCl3•6H2O) and two levels of phosphorus (P; 0 μM and 200 μM KH2PO4) combinations for 18 weeks, respectively. The proteome profile of Citrus root submitted to different P-Al interactions were investigated by using isobaric tags for relative and absolute quantification (iTRAQ).

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For origin melting Rep nucleoprotein complex accommodates both dsDNA and ssDNA of DUE

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Proteomic changes in liver of Channa striatus in response to high temperature stress

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Root adaptive responses to aluminum-toxicity revealed by RNA-Seq in two citrus species with contrasting aluminum tolerance

Project description:Background: Limited data are available on aluminum (Al)-toxicity-induced alterations of gene profiles in woody plants. Seedlings of Al-tolerant Citrus sinensis and Al-intolerant Citrus grandis were fertigated with nutrient solution containing 0 and 1.0 mM AlCl3•6H2O. Thereafter, we investigated the Al-toxicity-induced alterations of transcriptomics in roots by RNA-Seq. Results: Using RNA-seq, we isolated 1293 (990) up- and 1377 (915) downregulated genes from Al-treated C. grandis (C. sinensis) roots. Clearly, gene expression was less affected by Al-toxicity in C. sinensis roots than in C. grandis ones. Several Al-toxicity-responsive genes homologous to known Al-tolerance genes: Al-activated malate transporter, multidrug and toxic compound extrusion (MATE), IRON REGULATED/ferroportin 1, sensitive to proton rhizotoxicity 1 and monogalactosyldiacylglycerol synthase were identified in citrus roots. However, Al-induced upregulation of all these genes was stronger in C. grandis roots than in C. sinensis ones except for MATEs. Genes related to signal transduction, and sulfur transport and metabolism might also play a role in the higher Al-tolerance of C. sinensis. Conclusions: This is the first comparative investigation of transcriptomic responses in Al-treated citrus roots. There were common and unique mechanisms for citrus Al-tolerance. These results provide a platform for further investigating the roles of genes possibly responsible for citrus Al-tolerance.

2016-08-24 | GSE85958 | GEO

Root adaptive responses to aluminum-toxicity revealed by RNA-Seq in two citrus species with contrasting aluminum tolerance

Project description:Background: Limited data are available on aluminum (Al)-toxicity-induced alterations of gene profiles in woody plants. Seedlings of Al-tolerant Citrus sinensis and Al-intolerant Citrus grandis were fertigated with nutrient solution containing 0 and 1.0 mM AlCl3â?¢6H2O. Thereafter, we investigated the Al-toxicity-induced alterations of transcriptomics in roots by RNA-Seq. Results: Using RNA-seq, we isolated 1293 (990) up- and 1377 (915) downregulated genes from Al-treated C. grandis (C. sinensis) roots. Clearly, gene expression was less affected by Al-toxicity in C. sinensis roots than in C. grandis ones. Several Al-toxicity-responsive genes homologous to known Al-tolerance genes: Al-activated malate transporter, multidrug and toxic compound extrusion (MATE), IRON REGULATED/ferroportin 1, sensitive to proton rhizotoxicity 1 and monogalactosyldiacylglycerol synthase were identified in citrus roots. However, Al-induced upregulation of all these genes was stronger in C. grandis roots than in C. sinensis ones except for MATEs. Genes related to signal transduction, and sulfur transport and metabolism might also play a role in the higher Al-tolerance of C. sinensis. Conclusions: This is the first comparative investigation of transcriptomic responses in Al-treated citrus roots. There were common and unique mechanisms for citrus Al-tolerance. These results provide a platform for further investigating the roles of genes possibly responsible for citrus Al-tolerance. Examination of mRNA levels in control and Al-treatment roots of C. grandis and C. sinensis with two biological replicates were generated by deep sequencing, using Illumina HiSeq 2000 device.

2016-08-24 | E-GEOD-85958 | biostudies-arrayexpress

Leaf adaptive responses to aluminum-toxicity revealed by RNA-Seq in two citrus species with contrasting aluminum tolerance

Project description:Background: Limited data are available on aluminum (Al)-toxicity-induced alterations of gene profiles in woody plants. Seedlings of Al-tolerant Citrus sinensis and Al-intolerant Citrus grandis were fertigated with nutrient solution containing 0 and 1.0 mM AlCl3•6H2O. Thereafter, we investigated the Al-toxicity-induced alterations of transcriptomics in leaves by RNA-Seq. Results: Using RNA-seq, we isolated 1162 (181) up- and 496 (234) downregulated genes from Al-treated C. grandis (C. sinensis) leaves. Clearly, gene expression was less affected by Al-toxicity in C. sinensis leaves than in C. grandis ones. Several Al-toxicity-responsive genes homologous to known Al-tolerance genes: ALUMINUM SENSITIVE 3 (ALS3), multidrug and toxic compound extrusion (MATE), glutathione S-transferase (GST), L-galactose dehydrogenase（L-GalDH) and lipoxygenase (LOX) were identified in citrus leaves. Genes related to signal transduction, and sulfur transport and metabolism might also play a role in the higher Al-tolerance of C. sinensis. Conclusions: This is the first comparative investigation of transcriptomic responses in Al-treated citrus leaves. There were common and unique mechanisms for citrus Al-tolerance. These results provide a platform for further investigating the roles of genes possibly responsible for citrus Al-tolerance.

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