Dataset Information

ANKRD31 protein interactions

ABSTRACT: Sex chromosomes in males of most mammalian species share only a small homologous segment, the pseudoautosomal region (PAR), wherein double-strand break (DSB) formation must occur for correct meiotic segregation. We find that multiple DSB-promoting factors hyperaccumulate in the PAR, including the ankyrin-repeat domain-containing protein ANKRD31. We used immunoprecipitation and mass spectrometry from extracts of juvenile mouse testes to identify potential ANKRD31-interacting proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Male Gamete Generation

DISEASE(S): Disease Free

SUBMITTER: Zhuoning Li

LAB HEAD: Scott Keeney

PROVIDER: PXD017191 | Pride | 2020-06-19

REPOSITORIES: Pride

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Publications

Ensuring meiotic DNA break formation in the mouse pseudoautosomal region.

Acquaviva Laurent L Boekhout Michiel M Karasu Mehmet E ME Brick Kevin K Pratto Florencia F Li Tao T van Overbeek Megan M Kauppi Liisa L Camerini-Otero R Daniel RD Jasin Maria M Keeney Scott S

Nature 20200527 7812

Sex chromosomes in males of most eutherian mammals share only a small homologous segment, the pseudoautosomal region (PAR), in which the formation of double-strand breaks (DSBs), pairing and crossing over must occur for correct meiotic segregation<sup>1,2</sup>. How cells ensure that recombination occurs in the PAR is unknown. Here we present a dynamic ultrastructure of the PAR and identify controlling cis- and trans-acting factors that make the PAR the hottest segment for DSB formation in the m ...[more]

PMID: 32461690

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