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Development and validation of an ELISA to measure REG3E in canine blood

ABSTRACT: Regenerating islet-derived proteins (REG) are upregulated in people with sepsis, pancreatitis, and gastrointestinal diseases. One member of the REG family, namely REG3E, was recently identified in pancreatic tissue and plasma of dogs, with high expression in pancreatitis and sepsis. Our objective was to develop and validate an ELISA to measure REG3E concentrations in canine blood. An indirect sandwich ELISA was successfully developed using recombinant canine REG3E protein and polyclonal anti-canine REG3E antibodies raised in guinea pigs and rabbits. Antibody specificity was assessed using Western blot and mass spectrometric analysis of protein purified from canine plasma using Protein A Sepharose High Performance SpinTrap columns.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Plasma

DISEASE(S): Bacterial Sepsis,Pancreatitis

SUBMITTER: Manfred Heller

LAB HEAD: Laureen Peters

PROVIDER: PXD046936 | Pride | 2024-06-10

REPOSITORIES: Pride

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			Action	DRS
	20230925_S1_LP_i01.raw	Raw
	20230925_S1_LP_i02.raw	Raw
	20230925_S1_LP_i03.raw	Raw
	20230925_S1_LP_i04.raw	Raw
	20230925_S1_LP_i05.raw	Raw

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