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Protective impact on the apoptotic pathway by suppressing DMT1 during inflammatory stress

ABSTRACT: We performed phosphoproteomics using our sequential enrichment strategy of mRNA, protein, and phosphopeptides, which enabled us to explore the concurrent molecular events in the same sample and at the same time in normal and DMT1 silencing insulin-producing β-cells stimulated by siRNAs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)

SUBMITTER: Taewook Kang

LAB HEAD: Martin R. Larsen

PROVIDER: PXD017216 | Pride | 2021-08-10

REPOSITORIES: Pride

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			Action	DRS
	DMT1KD_Phosphoproteome.msf	Msf
	DMT1KD_Proteome.msf	Msf
	Replicate1_Flowthrough_1.raw	Raw
	Replicate1_Flowthrough_10.raw	Raw
	Replicate1_Flowthrough_11.raw	Raw

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Publications

Divalent Metal Transporter 1 Knock-Down Modulates IL-1β Mediated Pancreatic Beta-Cell Pro-Apoptotic Signaling Pathways through the Autophagic Machinery.

Kang Taewook T Huang Honggang H Mandrup-Poulsen Thomas T Larsen Martin R MR

International journal of molecular sciences 20210727 15

Pro-inflammatory cytokines promote cellular iron-import through enhanced divalent metal transporter-1 (DMT1) expression in pancreatic β-cells, consequently cell death. Inhibition of β-cell iron-import by DMT1 silencing protects against apoptosis in animal models of diabetes. However, how alterations of signaling networks contribute to the protective action of DMT1 knock-down is unknown. Here, we performed phosphoproteomics using our sequential enrichment strategy of mRNA, pr ...[more]

PMID: 34360779

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