Dataset Information

Mouse germ cell proteome from spermatogonia to round spermatids analysis by LC-MSMS

ABSTRACT: The data contains 135 raw data produced from mass spectrometry (QExactive HF, Thermo Scientific), the bio-samples were sperm cells collected at nine sub-stages of mouse spermatogenesis. Proteins from each bio-samples were extracted and digested to peptides, then separated to five fractions and triplicated analyzed by mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Qidan Li

LAB HEAD: Siqi Liu

PROVIDER: PXD017284 | Pride | 2020-12-01

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 136

Publications

Prediction and Validation of Mouse Meiosis-Essential Genes Based on Spermatogenesis Proteome Dynamics.

Fang Kailun K Li Qidan Q Wei Yu Y Zhou Changyang C Guo Wenhui W Shen Jiaqi J Wu Ruoxi R Ying Wenqin W Yu Lu L Zi Jin J Zhang Yuxing Y Yang Hui H Liu Siqi S Chen Charlie Degui CD

Molecular & cellular proteomics : MCP 20210106

The molecular mechanism associated with mammalian meiosis has yet to be fully explored, and one of the main reasons for this lack of exploration is that some meiosis-essential genes are still unknown. The profiling of gene expression during spermatogenesis has been performed in previous studies, yet few studies have aimed to find new functional genes. Since there is a huge gap between the number of genes that are able to be quantified and the number of genes that can be characterized by phenotyp ...[more]

PMID: 33257503

Similar Datasets

Project description:The birthrate following round spermatid injection (ROSI) remains low in current and evidence suggests that factors in the germinal vesicle (GV) cytoplasm and certain substances in the GV such as the nucleolus might be responsible for genomic reprogramming and embryonic development. However, little is known whether the reprogramming factors in GV oocyte cytoplasm and/or nucleolus in GV are beneficial to the reprogramming of round spermatids and development of ROSI embryos. Here, round spermatids were treated with GV cytolysates and injected this round spermatid alone or co-injected with GV oocyte nucleolus into mature metaphase II oocytes. Subsequent embryonic development was assessed morphologically and by Oct4 expression in blastocysts. There was no significant difference between experimental groups at the zygote to four-cell development stages. Blastocysts derived from oocytes which were injected with cytolysate treated-round spermatid alone or co-injected with nucleoli injection yielded 63.6% and 70.3% high quality embryos, respectively; comparable to blastocysts derived by intracytoplasmic sperm injection (ICSI), but higher than these oocytes which were co-injected with lysis buffer-treated round spermatids and nucleoli or injected with the lysis buffer-treated round spermatids alone. Furthermore, the proportion of live offspring resulting from oocytes which were co-injected with cytolysate treated-round spermatids and nucleoli or injected with cytolysate treated-round spermatids alone was higher than those were injected with lysis buffer treated-round spermaids, but comparable with the ICSI group. Our results demonstrate that factors from the GV cytoplasm improve round spermatid reprogramming, and while injection of the extra nucleolus does not obviously improve reprogramming its potential contribution, although which cannot be definitively excluded. Thus, some reprogramming factors are evidently present in GV oocyte cytoplasm and could significantly facilitate ROSI technology, while the nucleolus in GV seems also having a potential to improve reprogramming of round spermatids.

			Action	DRS
	Aun_F1_1.raw	Raw
	Aun_F1_2.raw	Raw
	Aun_F1_3.raw	Raw
	Aun_F2_1.raw	Raw
	Aun_F2_2.raw	Raw