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Dpb11 Immuno-Precipitation Mass Spectrometry (IPMS) Data Related to Mec1/ATR Signaling Upon Hyper-Resection

ABSTRACT: The Mec1/ATR kinase is crucial for genome maintenance, although the mechanisms by which it controls DNA repair pathways remain elusive. Here we uncovered a role for Mec1/ATR in controlling homologous recombination (HR) factors in response to hyper-resection of DNA ends. Cells lacking RAD9, a checkpoint activator and an inhibitor of resection, exhibit Mec1-dependent hyper-phosphorylation of proteins associated with single strand DNA transactions, including the ssDNA binding protein Rfa2, the translocase/ubiquitin ligase Uls1 and the HR-regulatory Sgs1-Top3-Rmi1 (STR) complex. Extensive Mec1-dependent phosphorylation of the STR complex, mostly on the Sgs1 helicase subunit, promotes an interaction between STR and the DNA repair scaffolding protein Dpb11. Fusion of Sgs1 to phosphopeptide-binding domains of Dpb11 strongly impairs HR-mediated repair, supporting a model whereby Mec1 promotes recruitment of STR to the 9-1-1 clamp via Dpb11 to regulate recombinogenic processes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Ethan Sanford

LAB HEAD: Marcus Smolka

PROVIDER: PXD017289 | Pride | 2021-03-10

REPOSITORIES: Pride

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			Action	DRS
	012918_R9delvsR9Ddc1del_HAIP_50pct_H1.mzXML	Mzxml
	012918_R9delvsR9Ddc1del_HAIP_50pct_H1.pep.xml	Pepxml
	012918_R9delvsR9Ddc1del_HAIP_50pct_H2.mzXML	Mzxml
	012918_R9delvsR9Ddc1del_HAIP_50pct_H2.pep.xml	Pepxml
	012918_R9delvsR9Ddc1del_HAIP_50pct_H3.mzXML	Mzxml

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