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Phosphoproteomic dataset comparing wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.

ABSTRACT: DNA double-strand breaks (DSBs) can be repaired by several pathways. In eukaryotes, repair pathway choice – the cellular decision making underlying DSB repair – occurs at the level of DSB resection and is controlled by the cell cycle. Upon cell cycle-dependent activation, cyclin-dependent kinases (CDKs) phosphorylate resection proteins and thereby stimulate DSB resection and repair by homologous recombination (HR). We uncovered a novel role for the Dbf4-dependent kinase Cdc7 (DDK) in regulating HR and DNA end resection. We therefore analyzed phosphorylation substrates of DDK by phosphoproteomics, where we compared wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Barbara Steigenberger

LAB HEAD: Prof. Dr. Boris Pfander

PROVIDER: PXD042607 | Pride | 2024-02-15

REPOSITORIES: Pride

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			Action	DRS
	20190718_coreQEP2_NaNa_LC12-17_SA_Orbi3256Sample09.raw	Raw
	20190718_coreQEP2_NaNa_LC12-17_SA_Orbi3256Sample10.raw	Raw
	20190718_coreQEP2_NaNa_LC12-17_SA_Orbi3256Sample11.raw	Raw
	20190718_coreQEP2_NaNa_LC12-17_SA_Orbi3256Sample12.raw	Raw
	20190718_coreQEP2_NaNa_LC12-17_SA_Orbi3256Sample13.raw	Raw

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