Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion

ABSTRACT: The centriole-to-centrosome conversion is a mechanism of the centrosome maturation process that depends on the formation of a proper centriole wall structure. Beside the centriole wall biogenesis factors TUBD1 and TUBE1, CEP44 is involved in this process. Lack of CEP44 generates defects in the centriole wall structure and thus, it interferes with the centrosome maturation. POC1B was found to be an interactor of CEP44 in the immunoprecipitation and LC-MS experiment. The interaction and the function of this hit was validated with further independent biological experiments.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: elmar schiebel

LAB HEAD: Elmar Johannes Schiebel

PROVIDER: PXD017332 | Pride | 2020-03-24

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	16-331_proteinGroups18.56.04.xlsx	Xlsx
	MaxQuant_Output.zip	Other
	Q25D165-331-01.raw	Raw

Items per page:

1 - 3 of 3

Publications

CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion.

Atorino Enrico S ES Hata Shoji S Funaya Charlotta C Neuner Annett A Schiebel Elmar E

Nature communications 20200214 1

Centrosomes are essential organelles with functions in microtubule organization that duplicate once per cell cycle. The first step of centrosome duplication is the daughter centriole formation followed by the pericentriolar material recruitment to this centriole. This maturation step was termed centriole-to-centrosome conversion. It was proposed that CEP295-dependent recruitment of pericentriolar proteins drives centriole conversion. Here we show, based on the analysis of proteins that promote c ...[more]

PMID: 32060285

Similar Datasets

The SON splicing factor is required for intracellular trafficking that promotes centriole assembly

Project description:Control of the number of centrosomes is critical for cell division, trafficking and cilia. Regulation of centrosome number occurs through the precise duplication of centrioles that reside in the center of centrosomes. Here we explored transcriptional control of centriole assembly by focusing on alternative splicing factors in isolation from other cell cycle processes. Of five splicing factors originally identified as required for centriole assembly, only SON is specifically required. Procentriole assembly is severely disrupted when SON is reduced but early centriole assembly events still occur. Whole genome mRNA sequencing identified thousands of genes whose splicing and expression are affected by the reduction of SON, with an enrichment of genes involved in the microtubule cytoskeleton. SON is required for the proper splicing and expression of the centriolar satellite protein, CEP131. Fluorescence microscopy and electron tomography establish key differences to the trafficking and microtubule network around the centrosomes that contribute to the centriole assembly defects. This establishes SON as required for centriole assembly, partially through its activity in splicing CEP131 and through control of microtubules organized by the centrosome.

2021-12-22 | GSE164278 | GEO

Single cell sequencing of the proliferative endometrium

Project description:Endometrial biopsies were collected on menstrual cycle day 7 from healthy fertile women age 24-42. Biopsies were processed to a single cell suspension and submitted for single cell sequencing on the 10x platform.

2021-09-18 | E-MTAB-10947 | biostudies-arrayexpress

RNAseq analysis of Xenopus laevis wildtype and emi2 mutant multiciliated cells during and post differentiation

Project description:Multiciliated cells (MCCs) form when progenitors massively expand centriole number, yielding the hundreds of basal bodies required to extend multiple motile cilia. This organelle biogenesis is promoted transcriptionally by Multicilin acting in a complex with the E2F transcription factors, which activates the expression of genes known to be involved required to form centrioles during the cell cycle, but also requires a cell cycle state required for the centrosome cycle. Multicilin also activates the expression of Emi2 (fbxo43) an inhibitor of the APC/C ubiquitin ligase. In Emi2 mutants, basal body formation during MCCl differentiation is blocked. RNAseq analysis is used to show that gene expression associated with MCC differentiation is upregulated normally in Emi2 mutants, but surprisingly is not downregulated when MCC differentiation is complete. Emi2 is required to promote the centrosomal cycle during MCC differentiation, but then also acts to turn off gene expression associated with centriole biogenesis in differentiated cells.

2024-03-20 | GSE195635 | GEO

RNA-seq of Pectobacterium carotovorum subsp. carotovorum and P. polaris infecting potato at 24, 48 and 72 hpi, and grown on M9 solid minimal medium at 24 hpi

Project description:Importance: Pectobacterium species cause soft rot in potato and other host plants primarily by secreting a battery of plant cell wall degrading enzymes. In addition, several different secretion systems are mobilized during infection. Previous studies of gene expression and regulation thereof primarily focused on the onset of infection. This work investigated transcriptome changes in Pectobacterium during the infection of potato tubers up to 72 hours post inoculation to elucidate biological processes during a longer infection period. Methods: The transcriptomes of aggressive strains of the two species P. carotovorum subsp. carotovorum and P. polaris were investigated during infection of potato minitubers (cv. 'Asterix') at 24, 48 and 72 hours after inoculation by RNA sequencing. The transcriptomes were compared to that of bacteria grown on minimal M9 medium, and transcriptomes from later infection time points (48 and 72 hours after inoculation) were compared to early infection (24 hours after inoculation). Results: Plant cell wall degrading enzymes and secretion system associated genes were largely upregulated during infection compared to in vitro growth, but downregulated in the later phases of infection compared to the early infection phase. The downregulation was not sufficiently explained by the expression of known virulence regulators such as the RsmA/B or the ExpA/S systems.

2022-04-01 | E-MTAB-7921 | biostudies-arrayexpress

Proteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method

Project description:Centrosomes are small cytoplasmic organelles that play fundamental roles in a range of cellular processes. Nearly all vertebrate cell types contain centrosomes, but whether centrosome composition and function differ between cell types, tissues and pathologies remains an outstanding question. Until now, probing centrosome composition has relied on proteomic profiling of centrosomes obtained by consecutive sucrose density gradient centrifugations, requiring up to one billion cells, thus rendering studies with multiple conditions cumbersome. Here we describe centrosome affinity capture (CAPture)-mass spectrometry (MS), a method that achieves high-coverage proteomes from 20-30 million cells. Utilising a biotin-labelled peptide derived from the CCDC61 protein, CAPture isolates intact centrosomes in a manner dependent on Ninein, a conserved centrosome component. CAPture-MS performs well across several untransformed and primary cell lines, thereby enabling comparisons of high-resolution centrosome proteomes. Using distal appendage proteins as an example, we demonstrate the utility of CAPture-MS for dissecting hierarchical interactions within the centrosome. Overall CAPture-MS represents a powerful tool to unveil temporal, regulatory, cell type- and tissue-specific changes in centrosome proteomes in health and disease.

2023-10-18 | PXD040308 | Pride

Mass-spectrometry based quantification of HYPK, NAA10 and NAA15 protein steady-state levels in Arabidopsis thaliana mutants carrying a T-DNA insertion in the HYPK gene.

Project description:N-alpha terminal acetylation is one of the major protein modifications in eukaryotes carried out by distinct Nats (N-alpha acetyltransferases). The core NatA complex is composed of the catalytic NAA10 and the auxiliary NAA15 subunit and co-translationally acetylates majority of the cytosolic proteins. HYPK interacts with NatA core complex in human and fungus in vivo and in vitro, regulating its activity. Here, a mass spectrometry approach was applied to quantify the steady-state levels of the core NatA subunits, NAA10 and NAA15 and of HYPK in the roots of Arabidopsis mutants carrying a T-DNA insertion in the HYPK gene (AT3G06610).

2022-08-12 | PXD023599 | Pride

RNA-seq of cells with centrosome amplification

Project description:Centrosome amplification induces proliferation arrest and cell invasion. The fate of centrosome amplification cells are studied here.

2021-10-07 | E-MTAB-11126 | biostudies-arrayexpress

RNA-seq of porcine peripheral blood mononuclear cells treated with lipopolysaccharide and dexamethasone

Project description:RNA-seq analysis was performed following the treatment of porcine PBMCs with lipopolysaccharide and dexamethasone for two hours to investigate responses to acute activation of immune and glucocorticoid receptor signaling and their crosstalk.

2021-11-30 | E-MTAB-9808 | biostudies-arrayexpress

Tuning the transcriptional response to hypoxia through HIF prolyl- and asparaginyl-hydroxylase inhibition: Microarray data

Project description:The hypoxia inducible factor (HIF) system orchestrates cellular responses to hypoxia in animals. HIF is an Î±/Î²-heterodimeric transcription factor that regulates the expression of hundreds of genes in a context dependent manner. A hypoxia-sensing component of the HIF system involves oxygen-dependent catalysis by the HIF hydroxylases; in humans there are three HIF prolyl hydroxylases (PHD1-3) and an asparaginyl hydroxylase (FIH). PHD catalysis regulates HIFÎ± levels and FIH catalysis regulates HIF activity. How differences in HIFÎ± hydroxylation status relate to variations in the induction of HIF target gene transcription is unknown. We report studies using small molecule inhibitors of the HIF hydroxylases to investigate the extent to which HIF target gene upregulation is induced by reduced PHD catalysis. The results reveal substantial differences in the role of prolyl- and asparaginyl-hydroxylation in regulating hypoxia responsive genes in cells. Selective PHD inhibitors with different structural scaffolds behave similarly. However, under the tested conditions, a broad-spectrum 2OG dioxygenase inhibitor is a better mimic of the transcriptional response to hypoxia than the selective PHD inhibitors, consistent with an important role for FIH in the hypoxic transcriptional response. Indeed, combined application of selective PHD and FIH inhibitors resulted in transcriptional induction of a subset of genes that were not fully responsive to PHD inhibition alone. Thus, for the therapeutic regulation of HIF target genes, it is important to consider both PHD and FIH activity, and in the case of some sets of target genes, simultaneous inhibition of the PHDs and FIH catalysis may be preferable.

2016-08-31 | E-MTAB-4300 | biostudies-arrayexpress

Functional genomic profiling of Aspergillus fumigatus biofilm reveals enhanced production of the mycotoxin gliotoxin

Project description:The opportunistic pathogenic mold Aspergillus fumigatus is an increasing cause of morbidity and mortality in immunocompromised and, in part, immunocompetent patients. Like bacteria or yeast, A. fumigatus can grow in multicellular communities by the formation of a hyphal network encased in an extracellular matrix. Here, we describe the proteome and transcriptome of planktonic and biofilm-grown A. fumigatus mycelium after 24h and 48h. A biofilm- and time-dependent regulation of many proteins and genes of the primary metabolism indicates a developmental stage of the young biofilm at 24h, which demands energy. At a matured biofilm phase, metabolic activity seems to be reduced. However, genes encoding hydrophobins and proteins involved in the biosynthesis of secondary metabolites were significantly upregulated. In particular, proteins of the gliotoxin secondary metabolite gene cluster were induced in biofilm cultures. This was confirmed by RT-PCR and by detection of this immunologically active mycotoxin in culture supernatants using HPLC analysis. The enhanced production of gliotoxin by in vitro formed biofilms reported here may play also a significant role under in vivo conditions. It may confer A. fumigatus protection from the host immune system and also enable its survival and persistence in chronic lung infections such as aspergilloma. Comparison of biofilm and submers cultures at 24h and 48h after induction.

2010-07-01 | E-GEOD-19430 | biostudies-arrayexpress