Dataset Information

TMT spectrometry unveils key M6a interactorsTMT spectrometry unveils key M6a interactors

ABSTRACT: The membrane glycoprotein M6a -together with proteolipid protein (PLP), DM20 and M6b- belongs to the tetraspan PLP family. M6a is a neuronal surface protein that promotes neuronal stem cell differentiation, migration, neurite and axonal outgrowth, filopodia/spine induction and synapse formation in primary neuronal cultures and non-neuronal cell lines. M6a has two extracellular loops (EC1 and EC2), four transmembrane domains, one intracellular loop and the N and C terminus facing the cell cytoplasm. However, the complete mechanism of action or proteins associated with it through its extracellular loops remained unknown. In previous work we provided strong evidence that M6aÃƒâ€šÃ‚Â´s extracellular loops widely contribute to its function. To asses this question, we designed and purified a chimera protein (M6a-loops as a bait protein) which was subjected to co-immunoprecipitation (Co-Ip) with rat hippocampi homogenates followed by TMT-MS. The TMT-MS and data analysis was done by the Proteomics Core Facility from the European Molecular Biology Laboratory (EMBL, Heidelberg, Germany).

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Camila Scorticati

LAB HEAD: Camila Scorticati

PROVIDER: PXD017347 | Pride | 2020-06-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Bert_181030_P0784_MR_CS_IP_1out10_R1.raw	Raw
	Bert_181109_P0784_MR_CS_IP_TMT_fr1.raw	Raw
	Bert_181109_P0784_MR_CS_IP_TMT_fr2.raw	Raw
	Bert_181109_P0784_MR_CS_IP_TMT_fr3.raw	Raw
	F075212.dat	Other

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Publications

Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS.

Aparicio Gabriela I GI Formoso Karina K León Antonella A Frasch Alberto C AC Scorticati Camila C

Frontiers in Synaptic Neuroscience 20200723

Nowadays, great efforts are made to gain insight into the molecular mechanisms that underlie structural neuronal plasticity. Moreover, the identification of signaling pathways involved in the development of psychiatric disorders aids the screening of possible therapeutic targets. Genetic variations or alterations in <i>GPM6A</i> expression are linked to neurological disorders such as schizophrenia, depression, and Alzheimer's disease. <i>GPM6A</i> encodes the neuronal surface glycoprotein M6a th ...[more]

PMID: 32848694

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