Dataset Information

Identification of cellular proteins binding to the 3’UTR of Programmed Cell Death 4 mRNA

ABSTRACT: The aim of the project is to identify RNA-binding proteins (RBPs) that interact with the 3’ untranslated region (3’UTR) of the programmed cell death 4 (PDCD4) mRNA. PDCD4 is an inflammatory tumor suppressor gene. The translation of PDCD4 mRNA is regulated by multiple RBPs. The aim is to identify RBPs which are critical for regulating PDCD4 mRNA translation by binding to its 3’UTR. For this purpose, a biotinylated PDCD4 3’UTR RNA was incubated with cytoplasmic lysate from MCF7 human breast carcinoma cells and the RNA-protein complexes pulled down by streptavidin affinity chromatography. The proteins were then eluted and resolved by SDS-PAGE. One particular protein band, migrating close to 55 KDa marker was cut out and submitted for proteomic analysis by in gel digestion and mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Chhaya Patole

LAB HEAD: Professor Partho Sarothi Ray

PROVIDER: PXD017376 | Pride | 2020-12-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	01012020_RID_1547_PDCD4_2.mgf	Mgf
	01012020_RID_1547_PDCD4_2.mgf-pride.xml.gz	Mgf
	01012020_RID_1547_PDCD4_2.pdResult	Other
	01012020_RID_1547_PDCD4_2.raw	Raw
	01012020_rid_1547_pdcd4_2-Final.xlsx	Xlsx

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Publications

RNA-binding proteins La and HuR cooperatively modulate translation repression of PDCD4 mRNA.

Kumar Ravi R Poria Dipak Kumar DK Ray Partho Sarothi PS

The Journal of biological chemistry 20201209

Posttranscriptional regulation of gene expression plays a critical role in controlling the inflammatory response. An uncontrolled inflammatory response results in chronic inflammation, often leading to tumorigenesis. Programmed cell death 4 (PDCD4) is a proinflammatory tumor-suppressor gene which helps to prevent the transition from chronic inflammation to cancer. PDCD4 mRNA translation is regulated by an interplay between the oncogenic microRNA miR-21 and the RNA-binding protein (RBP) human ant ...[more]

PMID: 33288677

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