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Aquatic decomposition and its effects on the bone proteome for forensic applications

ABSTRACT: Methods currently available to estimate the post-mortem submerged interval (PMSI) of cadavers in water suffer from poor accuracy, being mostly based on morphological examination of the remains. Proteins present within bones have recently attracted more attention from researchers interested in the estimation of the post-mortem interval (PMI) in terrestrial environments. Despite the great potential of proteomic methods for PMI estimation, their application to aquatic environments has not yet been explored. In this study, we examined whether four different types of aquatic environment (tap water, saltwater, pond water and chlorinated water) affected the proteome of mice bones with increasing PMSIs (from zero to three weeks).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Tibia

SUBMITTER: Noemi Procopio

LAB HEAD: Noemi Procopio

PROVIDER: PXD017386 | Pride | 2020-04-06

REPOSITORIES: Pride

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			Action	DRS
	NP_CM_1_1.mgf	Mgf
	NP_CM_1_1.mzid.gz	Mzid
	NP_CM_1_1.pride.mgf.gz	Mgf
	NP_CM_1_1.pride.mztab.gz	Mztab
	NP_CM_1_1.raw	Raw

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Publications

Aquatic Decomposition of Mammalian Corpses: A Forensic Proteomic Approach.

Mizukami Haruka H Hathway Bella B Procopio Noemi N

Journal of proteome research 20200416 5

Methods currently available to estimate the <i>postmortem</i> submerged interval (PMSI) of cadavers in water suffer from poor accuracy, being mostly based on morphological examination of the remains. Proteins present within bones have recently attracted more attention from researchers interested in the estimation of the <i>postmortem</i> interval (PMI) in terrestrial environments. Despite the great potential of proteomic methods for PMI estimation, their application to aquatic environments has n ...[more]

PMID: 32242669

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The implications of taphonomic alteration of human bone protein profiles for the development of a bone proteomics PMI/AAD estimation method.

Project description:The estimation of the post-mortem interval (PMI) and the age-at-death (AAD) are crucial steps in the medico-legal investigation of unidentified human remains. Current methods to estimate PMI and AAD suffer from a lack of accuracy and objectivity, particularly when the remains are in an advanced state of decomposition. Recently, proteomics studies using animal models have identified potential biomarkers for PMI and AAD estimation. This study investigated the human bone proteome in four human body donors studied throughout decomposition outdoors. We compared different bone tissues (tibia and iliac crest) from body donors of known AAD, and compared bone samples taken shortly after death to bone samples upon complete skeletonization of the body. The effects of ageing phenomena (in vivo and post-mortem) and the surrounding environment on the variability and abundancy of the bone proteome were assessed.

2021-09-09 | PXD019693 | Pride

Insights on the Differential Preservation of Bone Proteomes in Inhumated and Entombed Cadavers from Italian Forensic Caseworks

Project description:Bone is a long-lasting biological tissue often used in forensic investigations as it retains vital biomolecular information commonly used for identification purposes. Bone proteins have attracted interest for their potential in estimating post-mortem interval (PMI) and age-at-death (AAD). However, the preservation of such proteins is highly dependent on intrinsic and extrinsic factors, and these have an impact in the potential application of molecular techniques to forensic sciences. The present study aims at investigating the effect that two commonly used types of burial practices (entombment and inhumation) have on bone protein survival. The sample consists in 14 exhumed individuals from cemeteries in south of Italy at with different AADs (29-85 yeas) and PMIs (1-37 years). LC-MS/MS analyses show that 16 proteins are better preserved in the entombed condition and four in the inhumated one, while no clear cluster separation is detected with principal component analysis. Besides the different burial environments, several potential protein markers are identified for PMI and AAD estimation. Overall, preliminary results show that the two burial environments seem to play a marginal role in the differential preservation of non-collagenous proteins and in the accumulation of post-translational modifications, confirming the potential of LC-MS/MS based proteomics in forensic sciences.

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Expression data from murine cardiac tissue

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Bone Diagenesis in Short Timescales: Insights from an Explor-atory Proteomic Analysis

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