Dataset Information

Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants

ABSTRACT: The proteome of two sperm sources, epididymal and ejaculated sperm, was compared and associated with sperm freezing capacity using samples collected from three wild small ruminants species. Protein identification was performed using two databases separately (Ovis aries and Capra hircus NCBI) and results were later combined.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Ovis Aries Musimon Rupicapra Pyrenaica Capra Pyrenaica

TISSUE(S): Sperm

SUBMITTER: Marc Sylvester

LAB HEAD: Julian Santiago-Moreno

PROVIDER: PXD017462 | Pride | 2021-05-04

REPOSITORIES: Pride

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Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants.

Martínez-Fresneda Lucía L Sylvester Marc M Shakeri Farhad F Bunes Andreas A Del Pozo Juan C JC García-Vázquez Francisco A FA Neuhoff Christiane C Tesfaye Dawit D Schellander Karl K Santiago-Moreno Julian J

Cryobiology 20210121

Epididymal sperm shows higher cryoresistance than ejaculated sperm. Although the sperm proteome seems to affect cell cryoresistance, studies aiming at identifying proteins involved in sperm freezing-tolerance are scarce. The aims of this study were to investigate differences of sperm freezability and proteome between epididymal and ejaculated sperm in three mountain ungulates: Iberian ibex, Mouflon and Chamois. Sperm samples were cryopreserved in straws by slow freezing. Tandem mass tag-labeled ...[more]

PMID: 33485896

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