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SILAC-based quantitative proteomics analysis of prostate cancer cells

ABSTRACT: In this study, we applied SILAC-based quantitative proteomics analysis to identify hypoxia response proteome in prostate cancer cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Yue Chen

LAB HEAD: Yue Chen

PROVIDER: PXD017607 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	txt-du145-hypoxia.zip	Other
	yuechen_aluo_20190426_17314_MS_X_1_rerun.raw	Raw
	yuechen_aluo_20190426_17314_MS_X_2_rerun.raw	Raw
	yuechen_aluo_20190426_17314_MS_X_3.raw	Raw
	yuechen_aluo_20190426_17314_MS_X_4.raw	Raw

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Publications

Proteome dynamics analysis identifies functional roles of SDE2 and hypoxia in DNA damage response in prostate cancer cells.

Luo Ang A Gong Yao Y Kim Hyungjin H Chen Yue Y

NAR cancer 20200629 2

Mechanistic understanding of hypoxia-responsive signaling pathways provides important insights into oxygen- and metabolism-dependent cellular phenotypes in diseases. Using SILAC-based quantitative proteomics, we provided a quantitative map identifying over 6300 protein groups in response to hypoxia in prostate cancer cells and identified both canonical and novel cellular networks dynamically regulated under hypoxia. Particularly, we identified SDE2, a DNA stress response modulator, that was sign ...[more]

PMID: 32743553

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