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In-cell architecture of an active transcription-translation complex

ABSTRACT: Structural biology performed inside cells can capture molecular machines in action within their native context. Here we develop an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae. We combine whole-cell crosslinking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at sub-nanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpoint NusA at the interface between the ribosome and a NusG-bound elongating RNAP, and propose it could mediate transcription-translation coupling. Transcription inhibition stalls and rearranges the expressome, whereas translation inhibition dissociates it, demonstrating that the elongating expressome architecture requires active translation and transcription within the cell.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mycoplasma Pneumoniae (strain Atcc 29342 / M129)

SUBMITTER: Francis O'Reilly

LAB HEAD: Juri Rappsilber

PROVIDER: PXD017695 | Pride | 2020-07-30

REPOSITORIES: Pride

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			Action	DRS
	B171218_08_Lumos_LS_IN_120_MycoDSS_SCX09_hSAXfrac01_rep1.raw	Raw
	B171218_09_Lumos_LS_IN_120_MycoDSS_SCX09_hSAXfrac02_rep1.raw	Raw
	B171218_10_Lumos_LS_IN_120_MycoDSS_SCX09_hSAXfrac03_rep1.raw	Raw
	B171218_11_Lumos_LS_IN_120_MycoDSS_SCX09_hSAXfrac04_rep1.raw	Raw
	B171218_13_Lumos_LS_IN_120_MycoDSS_SCX09_hSAXfrac05_rep1.raw	Raw

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