Proteomics

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DICER-interacting partners in juvenile mouse testes

ABSTRACT: DICER has a well-characterized role in the processing of microRNAs (miRNAs) and small interfering RNAs (siRNA) that are important for post-transcriptional gene regulation. Emerging evidence suggests that DICER also has several non-canonical functions beyond miRNA/siRNA biogenesis, for example in transcriptional gene silencing at the chromatin level, as well as in RNA degradation and maintenance of genomic integrity. We have shown that the function of DICER in germ cells is essential for normal spermatogenesis; male mice lacking DICER in postnatal male germ cells are infertile due to severe defects in haploid differentiation. To better understand the function of DICER in male germ cells, we immunoprecipitated DICER from juvenile mouse testes and performed mass spectrometric analysis to identify DICER-interacting proteins.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Ram Prakash Yadav  

LAB HEAD: Noora Kotaja

PROVIDER: PXD017772 | Pride | 2020-06-03

REPOSITORIES: Pride

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Action DRS
180816_dicer_IP1.msf Msf
180816_dicer_IP1.msfView Msf
180816_dicer_IP1.pdResult Other
180816_dicer_IP1.pdResultView Other
180816_dicer_IP1.raw Raw
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Publications

DICER regulates the expression of major satellite repeat transcripts and meiotic chromosome segregation during spermatogenesis.

Yadav Ram Prakash RP   Mäkelä Juho-Antti JA   Hyssälä Hanna H   Cisneros-Montalvo Sheyla S   Kotaja Noora N  

Nucleic acids research 20200701 13

Constitutive heterochromatin at the pericentric regions of chromosomes undergoes dynamic changes in its epigenetic and spatial organization during spermatogenesis. Accurate control of pericentric heterochromatin is required for meiotic cell divisions and production of fertile and epigenetically intact spermatozoa. In this study, we demonstrate that pericentric heterochromatin is expressed during mouse spermatogenesis to produce major satellite repeat (MSR) transcripts. We show that the endonucle  ...[more]

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