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Novel Proteome Extraction Method Illustrate a Conserved Immunological Signature of MSI-H Colorectal Tumors

ABSTRACT: Utilizing the novel TOP clinical proteome extraction to better understand the immune phenotype of MSI-H CRC tumors

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Gilad Vainer

LAB HEAD: Gilad Vainer

PROVIDER: PXD017821 | Pride | 2020-07-10

REPOSITORIES: Pride

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			Action	DRS
	OxidationMSites.txt	Txt
	QEP2_GVAI_2_00750_1_230316.raw	Raw
	QEP2_GVAI_2_00750_2_230316.raw	Raw
	QEP2_GVAI_2_01563_10_1_230316.raw	Raw
	QEP2_GVAI_2_02301_4_1_230316.raw	Raw

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Novel Proteome Extraction Method Illustrates a Conserved Immunological Signature of MSI-H Colorectal Tumors.

Vainer Elez D ED Kania-Almog Juliane J Zatara Ghadeer G Levin Yishai Y Vainer Gilad W GW

Molecular & cellular proteomics : MCP 20200708 10

Using a simple, environment friendly proteome extraction (TOP), we were able to optimize the analysis of clinical samples. Using our TOP method we analyzed a clinical cohort of microsatellite stable (MSS) and unstable (MSI-H) colorectal carcinoma (CRC). We identified a tumor cell specific, STAT1-centered, immune signature expressed by the MSI-H tumor cells. We then showed that long, but not short, exposure to Interferon-γ induces a similar signature <i>in vitro</i> We identified 10 different tem ...[more]

PMID: 32641473

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Novel Proteome Extraction Method Illustrate a Conserved Immunological Signature of MSI-H Colorectal Tumors

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