Proteomics

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Identification of interactors of Sth1 in Candida albicans

ABSTRACT: Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. To precisely determine the composition of the CaRSC complex, we immunopurified Myc-tagged Sth1, the core catalytic subunit of the CaRSC complex encoded by C3_02490C and identified its physical interactors using high-resolution tandem mass spectrometry (LC-MS/MS). Wild-type cells expressing untagged Sth1 served as negative control, and all immunoprecipitation experiments were analyzed in biological triplicates. No antibody controls for immunopurification were also analysed.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Vinutha K B  

LAB HEAD: Santanu Kumar Ghosh

PROVIDER: PXD017988 | Pride | 2020-11-11

REPOSITORIES: Pride

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Publications

The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits.

Balachandra Vinutha K VK   Verma Jiyoti J   Shankar Madhu M   Tucey Timothy M TM   Traven Ana A   Schittenhelm Ralf B RB   Ghosh Santanu K SK  

PLoS genetics 20201105 11

Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. Biochemical analysis showed that CaRSC comprises 13 subunits and contains two novel non-essential members, which we named Nri1 and Nri2 (Novel RSC Interactors) that are exclusive to the CTG clade of  ...[more]

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