Dataset Information

Validation of Nri1/Nri2 physical interaction with the Candida albicans RSC complex

ABSTRACT: Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. In this study, we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans (CaRSC). To precisely determine the composition of the CaRSC complex, we immunopurified Myc-tagged Sth1, the core catalytic subunit of the CaRSC complex encoded by C3_02490C and identified its physical interactors using high-resolution tandem mass spectrometry (LC-MS/MS). Strikingly, we identified two novel hitherto uncharacterized proteins that co-purified with Sth1, C2_01370p (Nri1) and C1_01800p (Nri2), whose homologs are annotated only in the CTG clade of fungi. Here, we performed reciprocal co-immunoprecipitations of Myc-tagged Nri1 and Nri2 followed by LC-MS/MS to validate these interactions. For each condition, biological duplicates were analyzed.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Vinutha K B

LAB HEAD: Santanu Kumar Ghosh

PROVIDER: PXD017987 | Pride | 2020-11-11

REPOSITORIES: Pride

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			Action	DRS
	P2RS20180613_10Eminus.raw	Raw
	P2RS20180613_10Eminus.raw_20190520_Byonic.xlsx	Xlsx
	P2RS20180613_11Eplus.raw	Raw
	P2RS20180613_11Eplus.raw_20190520_Byonic.xlsx	Xlsx
	P2RS20180613_12Eminus.raw	Raw

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Publications

The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits.

Balachandra Vinutha K VK Verma Jiyoti J Shankar Madhu M Tucey Timothy M TM Traven Ana A Schittenhelm Ralf B RB Ghosh Santanu K SK

PLoS genetics 20201105 11

Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. Biochemical analysis showed that CaRSC comprises 13 subunits and contains two novel non-essential members, which we named Nri1 and Nri2 (Novel RSC Interactors) that are exclusive to the CTG clade of ...[more]

PMID: 33151931

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