Dataset Information

ChIP-MS of Glucocorticoid Receptor in primary mouse macrophages

ABSTRACT: Glucocorticoids (GCs) are the most effective anti-inflammatory drugs in current clinical settings, yet their genomic modes of action are poorly understood. GCs bind to the Glucocorticoid Receptor (GR), which acts as a transcription factor to control gene expression in the immune system. Understanding the molecular mechanism that delineates gene repression of pro-inflammatory target genes from gene activation of metabolic genes will help to improve GC therapy and overcome adverse effects.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Michael Wierer

LAB HEAD: Matthias Mann

PROVIDER: PXD018077 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20150119_QEp3_MiWi_SA_Franziska_GR_1.raw	Raw
	20150119_QEp3_MiWi_SA_Franziska_GR_2.raw	Raw
	20150119_QEp3_MiWi_SA_Franziska_GR_3.raw	Raw
	20150119_QEp3_MiWi_SA_Franziska_IgG_1.raw	Raw
	20150119_QEp3_MiWi_SA_Franziska_IgG_2.raw	Raw

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Publications

The glucocorticoid receptor recruits the COMPASS complex to regulate inflammatory transcription at macrophage enhancers.

Greulich Franziska F Wierer Michael M Mechtidou Aikaterini A Gonzalez-Garcia Omar O Uhlenhaut N Henriette NH

Cell reports 20210201 6

Glucocorticoids (GCs) are effective anti-inflammatory drugs; yet, their mechanisms of action are poorly understood. GCs bind to the glucocorticoid receptor (GR), a ligand-gated transcription factor controlling gene expression in numerous cell types. Here, we characterize GR's protein interactome and find the SETD1A (SET domain containing 1A)/COMPASS (complex of proteins associated with Set1) histone H3 lysine 4 (H3K4) methyltransferase complex highly enriched in activated mouse macrophages. We s ...[more]

PMID: 33567280

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