Dataset Information

Exploring the Wound Healing, Anti-Inflammatory, Anti-Pathogens and Proteomic Effects of Lactic Acid Bacteria on Keratinocytes

ABSTRACT: The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogen effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Marcello Manfredi

LAB HEAD: Marcello Manfredi

PROVIDER: PXD018088 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CecconiHacat_CTRLA_28012020_LIB1.dat	Other
	CecconiHacat_CTRLA_28012020_LIB2.dat	Other
	DDAWifffiles.zip	Other
	SASGL04_HACAT_1UG_03012020_LIB1.dat	Other
	SASGL07_HACAT_1UG_03012020_LIB1.dat	Other

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Publications

Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes.

Brandi Jessica J Cheri Samuele S Manfredi Marcello M Di Carlo Claudia C Vita Vanella Virginia V Federici Federica F Bombiero Eleonora E Bazaj Alda A Rizzi Eleonora E Manna Laura L Cornaglia Giuseppe G Marini Umberto U Valenti Maria Teresa MT Marengo Emilio E Cecconi Daniela D

Scientific reports 20200714 1

The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization ...[more]

PMID: 32665600

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