Proteomics

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Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2.


ABSTRACT: MetQ gene of Streptococcus suis serotype 2 deletion strain has attenuated antiphagocytosis. However,the mechanism of antiphagocytosis and pathogenesis of MetQ in SS2 has remained unclear. In this study, stable isotope labeling by amino acids in cell culture (SILAC) based liquid chromatography-mass spectrometry (LC-MS) and subsequent bioinformatics analysis was used to determine differentially expressed proteins of RAW264.7 cells infected with △MetQ and ZY05719, aimed at elucidating the mechanism of antiphagocytosis and innate immunity of macrophages infected by Streptococcus suis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Acute Leukemia

SUBMITTER: Xiaomeng Pei  

LAB HEAD: Hongjie Fan

PROVIDER: PXD018091 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

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Action DRS
P17655_A_1_1.mgf Mgf
P17655_A_1_1.raw Raw
P17655_A_1_2.mgf Mgf
P17655_A_1_2.raw Raw
P17655_A_1_3.mgf Mgf
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Publications

Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2.

Pei Xiaomeng X   Liu Junchi J   Liu Mingxing M   Zhou Hong H   Wang Xiaomin X   Fan Hongjie H  

AMB Express 20201030 1


Streptococcus suis serotype 2 (SS2) is a serious zoonotic pathogen; it can lead to symptoms of streptococcal toxic shock syndrome (STSS) in humans and sepsis in pigs, and poses a great threat to public health. The SS2 MetQ gene deletion strain has attenuated antiphagocytosis, although the mechanism of antiphagocytosis and pathogenesis of MetQ in SS2 has remained unclear. In this study, stable isotope labeling by amino acids in cell culture (SILAC) based liquid chromatography-mass spectrometry (L  ...[more]

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